A freeze-thawing method to prepare chitosan-poly(Vinyl alcohol) hydrogels without crosslinking agents and diflunisal release studies

María Dolores Figueroa-Pizano, Itziar Vélaz*, María Elisa Martínez-Barbosa

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Chitosan-poly(vinyl alcohol) hydrogels can be produced by the freeze-thawing method without using toxic crosslinking agents. The applications of these systems are limited by their characteristics (e.g., porosity, flexibility, swelling capacity, drug loading and drug release capacity), which depend on the freezing conditions and the kind and ratio of polymers. This protocol describes how to prepare hydrogels from chitosan and poly(vinyl alcohol) at 50/50 w/w % of polymer composition and varying the freezing temperature (-4 °C,-20 °C,-80 °C) and freeze-thawing cycles (4, 5, 6 freezing cycles). FT-IR spectra, SEM micrograph and porosimetry data of hydrogels were obtained. Also, the swelling capacity and drug loading and release of diflunisal were assessed. Results from SEM micrographs and porosimetry show that the pore size decreases, while the porosity increases at lower temperatures. The swelling percentage was higher at the minor freezing temperature. The release of diflunisal from the hydrogels has been studied. All the networks maintain the drug release for 30 h and it has been observed that a simple diffusion mechanism regulates the diflunisal release according to Korsmeyer-Peppas and Higuchi models.

Original languageEnglish
Article numbere59636
JournalJournal of Visualized Experiments
Volume2020
Issue number155
DOIs
StatePublished - Jan 2019

Bibliographical note

Publisher Copyright:
© 2020 Journal of Visualized Experiments.

Keywords

  • Bioengineering
  • Chitosan-Poly(vinyl alcohol) hydrogels
  • Diflunisal
  • Drug loading and release studies
  • Freeze-thawing
  • Issue 155
  • Network characterization
  • Porosimetry

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