A potential inflammatory role of IL-31 in psoriatic arthritis: A correlation with Th17 cytokine profile

L. A. Bautista-Herrera, U. De la Cruz-Mosso, I. V. Román-Fernández, I. Parra-Rojas, J. G. Soñanez-Organis, J. Hernández-Bello, R. A. Morales-Zambrano, G. D. Villanueva-Quintero, J. F. Muñoz-Valle*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The goals of our study were to determine the possible association of interleukin (IL)-31 with Th17 cytokine profile in serum and to quantify retinoic acid-related orphan receptor C (RORC) mRNA expression in psoriatic arthritis (PsA) patients. This cross-sectional study was conducted in 50 patients with PsA and 30 control subjects (CS) matched by age and gender. The cytokine serum levels were quantified by magnetic bead–based assay using the Bio-Plex MAGPIX system, and RORC mRNA expression was determined by quantitative polymerase chain reaction (qPCR). As a result, significant differences in IL-31 were observed between study groups (77.23 pg/mL in PsA vs 64.4 pg/mL in CS, P < 0.001) and Th17 cytokine profile serum levels (IL-17A: 6.36 pg/mL in PsA vs 2.97 pg/mL in CS, P = 0.02; IL-17F: 44.15 pg/mL in PsA vs 23.36 pg/mL in PsA, P = 0.01; IL-17E: 3.03 pg/mL in PsA vs 0.82 pg/mL in CS, P < 0.001; IL-21: 36.45 pg/mL in PsA vs 12.44 pg/mL in CS, P = 0.02); however, significant differences were not observed for IL-23 (31.2 pg/mL in PsA vs 53.26 pg/mL in CS, P = 0.58). Furthermore, positive correlations between IL-31 and Th17 cytokine profile serum levels were found (IL-17A: rs = 0.64, P < 0.001; IL-17F: rs = 0.73, P < 0.001; IL-17E: rs = 0.70, P < 0.001; IL-21: rs = 0.54, P = 0.002; IL-23: rs = 0.5, P < 0.01). Regarding RORC gene expression, the PsA group showed an increase of 6.85-fold compared to the CS group. We did not find any association between the serum levels of cytokines and RORC gene expression. In conclusion, in PsA, there are increased serum levels of IL-31, IL-17A, IL-17F, IL-17E, and IL-21, but not IL-23. Moreover, there was a positive correlation of IL-31 with the Th17 cytokine profile and a high RORC gene expression. Altogether, these findings suggest a proinflammatory contribution of IL-31 in close association with the Th17 cytokine profile in PsA.

Original languageEnglish
JournalInternational Journal of Immunopathology and Pharmacology
Volume34
DOIs
StatePublished - 2020

Bibliographical note

Funding Information:
The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by funding from Universidad de Guadalajara through Fortalecimiento de la Investigación y el Posgrado 2018 Grant (No. 244159), assigned to J.F.M.V.

Publisher Copyright:
© The Author(s) 2020.

Keywords

  • IL-31
  • RORC
  • Th17 cytokine profile
  • mRNA expression
  • psoriatic arthritis

Fingerprint

Dive into the research topics of 'A potential inflammatory role of IL-31 in psoriatic arthritis: A correlation with Th17 cytokine profile'. Together they form a unique fingerprint.

Cite this