Activity of chitosan–lysozyme nanoparticles on the growth, membrane integrity, and β-1,3-glucanase production by Aspergillus parasiticus

Cynthia Nazareth Hernández-Téllez, Francisco Julián Rodríguez-Córdova, Ema Carina Rosas-Burgos, Mario Onofre Cortez-Rocha, Armando Burgos-Hernández, Jaime Lizardi-Mendoza, Wilfrido Torres-Arreola, Aarón Martínez-Higuera, Maribel Plascencia-Jatomea*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Synthesis of nanocomposites from antimicrobial biopolymers such as chitosan (CS) and lysozyme (LZ) is an important and promising area in bionanotechnology. Chitosan–lysozyme (CS–LZ) nanoparticles (NPs) were prepared by the nanoprecipitation method, using commercial chitosan of 153 kDa. TEM and dynamic light scattering (DLS) analysis were carried out to evaluate the morphology, size, dispersion, and Z potential. Association efficiency of lysozyme was determined using Coomassie blue assay. The antifungal activity of NPs against Aspergillus parasiticus was evaluated through cell viability (XTT), germination and morphometry of spores, and reducing sugars production; the effects on membrane integrity and cell wall were also analyzed. NPs’ size were found in the range of 13.4 and 11.8 nm for CS–LZ and CS NPs, respectively, and high Z potential value was observed in both NPs. Also, high association of lysozyme was presented in the CS matrix. With respect to the biological responses, CS–LZ NPs reduced the viability of A. parasiticus and a strong inhibitory effect on the germination of spores (100% of inhibition) was observed at 24 h in in vitro assays. CS–LZ and CS NPs affected the membrane integrity and the cell wall of spores of fungi with respect to control, which is consistent with the low amount of reducing sugars detected. CS–LZ NPs prepared by nanoprecipitation promise to be a viable and safe alternative for use in biological systems, with a possible low or null impact to humans and biota. However, the potential benefits and the environmental and health implications of NPs need to be globally discussed due to its possible negative effects.

Original languageEnglish
Article number279
Journal3 Biotech
Volume7
Issue number5
DOIs
StatePublished - 1 Oct 2017

Bibliographical note

Publisher Copyright:
© 2017, Springer-Verlag GmbH Germany.

Keywords

  • Aspergillus parasiticus
  • Cell viability
  • Cell wall
  • Chitosan–lysozyme nanocomposites
  • Membrane integrity
  • Morphometry
  • Nanoparticles
  • β-1,3-Glucanase

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