Aging does not affect calcium response to CCL2 and LPS in human monocytes

Bruno de Jesús Arellano-Cruz, María de los Ángeles Vázquez-Prieto, Nilda Belén Fernández-Eufrasio, Dvorak Montiel-Condado, Genaro Patiño-López, Adriana Garibay-Escobar, Adriana Sumoza-Toledo*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Monocytes play important roles in anti-microbial and anti-viral responses and chronic inflammatory diseases. Monocytes' functions are altered by aging. We investigated age-changes in calcium (Ca2+) response to CCL2 and LPS in human monocytes. CCL2 and LPS induced a slow increase of the cytosolic Ca2+ level, with a maximum response at ∼360 s and ∼300 s, respectively, in monocytes of young and older adults. No difference was observed in the magnitude and in the Ca2+ kinetic with both stimuli. Furthermore, store-operated Ca2+ entry and plasma membrane expression of ORAI1 showed no difference between both groups. In summary, monocytes from older adults maintained the capacity to mobilize calcium as their counterparts in young adults suggesting that the mechanisms underlying the dysfunctions in monocytes in aging might not involve alterations in Ca2+ flow through the plasma membrane.

Original languageEnglish
JournalHuman Immunology
DOIs
StateAccepted/In press - 2021
Externally publishedYes

Bibliographical note

Funding Information:
We thank Universidad Veracruzana for the financial assistance to perform this work and the Consejo Nacional de Ciencia y Tecnología (CONACYT) with grant number 255035.

Publisher Copyright:
© 2021 American Society for Histocompatibility and Immunogenetics

Keywords

  • Aging
  • Calcium
  • Monocytes
  • ORAI1
  • SOCE

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