Analysis of free amino acids in fermented shrimp waste by high-performance liquid chromatography

J. López-Cervantes, D. I. Sánchez-Machado, J. A. Rosas-Rodríguez

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92 Scopus citations


This work presents an HPLC method for the quantification of free amino acids in lyophilized protein fraction from shrimp waste hydrolysate which is obtained by acid lactic fermentation and analyzed using pre-column derivatization with 9-fluorenylmethyl-chloroformate. The amino acids were separated in a Hypersil ODS 5 μm column (250 mm × 4.6 mm) at 38°C. The mobile phase was a mixture of phase A: 30 mM ammonium phosphate (pH 6.5) in 15:85 (v/v) methanol/water; phase B: 15:85 (v/v) methanol/water; and phase C: 90:10 (v/v) acetonitrile/water, with flow rate 1.2 ml/min. Fluorescence detection was used at an excitation wavelength of 270 nm and an emission wavelength of 316 nm. Method precisions for the different amino acids were between 4.4 and 7.1% (relative standard deviation, RSD); detection limits were between 23 and 72 ng/ml; and the recoveries were between 89.0 and 95.0%. The amino acid present at the highest concentration was tyrosine. © 2005 Elsevier B.V. All rights reserved.
Original languageAmerican English
Pages (from-to)106-110
Number of pages5
JournalJournal of Chromatography A
Issue number1-2 SPEC. ISS.
StatePublished - 10 Feb 2006
Externally publishedYes
EventJournal of Chromatography A -
Duration: 10 Feb 2006 → …

Bibliographical note

Funding Information:
This work was financed under project no. ITSON-EXB-36 from the Program of Profesor's Improvement from the Public Education Department (PROMEP). Michael A. Bryan, MBA and Native English Professor, offered suggestions and improvements in the wording of this manuscript.


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