Antimutagenic, antiproliferative and antioxidant properties of sea grape leaf extract fractions (Coccoloba uvifera l.)

Jorge A. Ramos-Hernández, Montserrat Calderón-Santoyo, Armando Burgos-Hernández, Joel S. García-Romo, Arturo Navarro-Ocaña, María G. Burboa-Zazueta, Edgar Sandoval-Petris, Juan A. Ragazzo-Sánchez*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Background: Cancer is a disease characterized by the invasion and uncontrolled growth of cells. One of the best ways to minimize the harmful effects of mutagens is through the use of natural antimutagens. In this regard, the search for new antimutagens that act in the chemoprevention could represent a promising field in this area. Objective: In this study biological potential of 11 fractions from Coccoloba uvifera L. leaf hexane extract was evaluated by several in vitro tests. Methods: Leaves were lyophilized and hexane extraction was performed. The extract was fractionated by col-umn chromatography with hexane, ethyl acetate, and methanol. The antimutagenic (Ames test), antiprolifera-tive (MTT test), and antioxidant capacity (DPPH, ABTS, and ferrous ion chelation) of the fractions were evalu-ated. Results: Fractions 4, 6, 8, and 9 have antimutagenic activity (against sodium azide in strain TA100), fraction 11 showed antiproliferative capacity (IC50 of 24 ± 9 μg/mL in cells of HCT 116). The fractions with the highest activity were analyzed by HPLC-MS and lupeol, acacetin, and β-sitosterol were identified. Conclusion: This study demonstrates, for the first time, the bioactivity of C. uvifera leaf as a new source of High Biological Value Compounds (HBVC), which can be of interest to the food and pharmaceutical indus-tries.

Original languageEnglish
Pages (from-to)2250-2257
Number of pages8
JournalAnti-Cancer Agents in Medicinal Chemistry
Volume21
Issue number16
DOIs
StatePublished - Nov 2021

Bibliographical note

Funding Information:
This study was supported by the Consejo Nacional de Ciencia y Tecnoligía (CONACyT, Mexico) for the scholarship granted (number 577058) to Jorge Alberto Ramos Hernández and the CYT-ED thematic network code 319RT0576.

Funding Information:
This study was supported by the Consejo Nacional de Ciencia y Tecnolig?a (CONACyT, Mexico) for the scholarship granted (number 577058) to Jorge Alberto Ramos Hern?ndez and the CYTED thematic network code 319RT0576.

Publisher Copyright:
© 2021 Bentham Science Publishers.

Keywords

  • Antimutagenicity
  • Antioxidant
  • Antiproliferation
  • Coccoloba uvifera leaf
  • Fractions
  • Hexane extract

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