Antimutagenic bis (2-ethylhexyl) phthalate isolated from octopus (paraoctopus vulgaris)

Susana Gabriela Cruz-Ramírez, Carmen María López-Saiz, Ema Carina Rosas-Burgos, Francisco Javier Cinco-Moroyoqui, Carlos Velázquez, Javier Hernández, Armando Burgos-Hernández*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


In the present study, the antimutagenic activity of methanolic (MP) and hexanic (HP) phase from hexane-soluble extract of Paraoctopus vulgaris was evaluated. The HP showed significant concentration-dependent antimutagenic activity (p < 0.05) in the Ames assay against aflatoxin B1 (AFB1 ) using Salmonella typhimurium TA98 and TA100 tester strains. Seven fractions (F1-F7) were obtained from HP by silica gel-column chromatography and the antimutagenic activity of F6 and F7 caused significant reduction (p < 0.05) in the number of AFB1-induced revertants in both strains. However, high antimutagenic activity was exhibited in TA98 strain (85-97% reversion inhibition) and remained at a fraction-concentration of 3 μg/mL. F6 and F7 composition was analyzed by high-performance liquid chromatography/photodiode array, both fractions shared one peak at 9.42 min suggesting presence of the same compound.1H-NMR and13C-NMR analysis results indicate that the bioactive compound is bis (2-ethylhexyl) phthalate. Based on of these results bis (2-ethylhexyl) phthalate exhibit antimutagenic activity, which allows to consider it for further investigation as a potential chemopreventive compound.

Original languageEnglish
Pages (from-to)314-320
Number of pages7
JournalFood Science and Technology
Issue number2
StatePublished - 1 Apr 2021

Bibliographical note

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© 2021, Sociedade Brasileira de Ciencia e Tecnologia de Alimentos, SBCTA. All rights reserved.


  • Antimutagenic
  • Bis (2-ethylhexyl) phthalate
  • Chemopreventive
  • Paraoctopus vulgaris


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