Antimycobacterial Precatorin A Flavonoid Displays Antibiofilm Activity against Mycobacterium bovis BCG

Esmeralda Ivonne Niño-Padilla, Clara Espitia, Carlos Velazquez, Efrain Alday, Erika Silva-Campa, Alexel Burgara-Estrella, José Antonio Enciso-Moreno, Olivia Valenzuela, Humberto Astiazarán-García, Adriana Garibay-Escobar*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


The aim of this study was to evaluate the potential antibiofilm activity of Rhynchosia precatoria (R. precatoria) compounds over Mycobacterium bovis BCG (M. bovis BCG) as a model for Mycobacterium tuberculosis (Mtb). We evaluated the antibiofilm activity as the ability to both inhibit biofilm formation and disrupt preformed biofilms (bactericidal) of R. precatoria compounds, which have been previously described as being antimycobacterials against Mtb. M. bovis BCG developed air-liquid interface biofilms with surface attachment ability and drug tolerance. Of the R. precatoria extracts and compounds that were tested, precatorin A (PreA) displayed the best biofilm inhibitory activity, as evaluated by biofilm biomass quantification, viable cell count, and confocal and atomic force microscopy procedures. Furthermore, its combination with isoniazid at subinhibitory concentrations inhibited M. bovis BCG biofilm formation. Nonetheless, neither PreA nor the extract showed bactericidal effects. PreA is the R. precatoria compound responsible for biofilm inhibitory activity against M. bovis BCG.

Original languageEnglish
Pages (from-to)40665-40676
Number of pages12
JournalACS Omega
Issue number43
StatePublished - 31 Oct 2023

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© 2023 The Authors. Published by American Chemical Society.


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