Antioxidant Activity of Peptide Fractions from Chickpea Globulin Obtained by Pulsed Ultrasound Pretreatment

María Fernanda González-Osuna; Wilfrido Torres-Arreola; Enrique Márquez-Ríos; Francisco Javier Wong-Corral; Eugenia Lugo-Cervantes; José Carlos Rodríguez-Figueroa; Guillermina García-Sánchez; Josafat Marina Ezquerra-Brauer; Herlinda Soto-Valdez; Alejandro Castillo; Carmen Lizette Del-Toro-Sánchez

doi:10.3390/horticulturae9040415

Antioxidant Activity of Peptide Fractions from Chickpea Globulin Obtained by Pulsed Ultrasound Pretreatment

María Fernanda González-Osuna, Wilfrido Torres-Arreola, Enrique Márquez-Ríos, Francisco Javier Wong-Corral, Eugenia Lugo-Cervantes, José Carlos Rodríguez-Figueroa, Guillermina García-Sánchez, Josafat Marina Ezquerra-Brauer, Herlinda Soto-Valdez, Alejandro Castillo, Carmen Lizette Del-Toro-Sánchez^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Protein hydrolysates and peptides can show biological activities, and pulsed ultrasound improves bioactivities. Among matrices from which protein hydrolysates can be obtain, chickpea is an excellent source. The objective of this research was to evaluate the effect of pulsed ultrasound on globulin concentrate to obtain chickpea hydrolysate (HGb) and peptide fractions and their bioactivity. Antioxidant activity by ABTS (2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt), FRAP (Ferric Reducing Antioxidant Power) and human erythrocyte assays was determined. The electrophoretic profile, amino acid profile, and antimicrobial activity of hydrolysates were also determined. Two hydrolysates had the highest antioxidant activity: HGb (91.44% ABTS inhibition, 73.04% hemolysis inhibition and 5185.57 µmol TE/g dried sample in FRAP assay) and HGb-20 (48.25% ABTS inhibition, 100% hemolysis inhibition and 2188.53 µmol TE/g dried sample in FRAP assay). Peptide fractions inhibited 100% of the hemolysis on human erythrocytes. The hydrolysates from chickpea proteins obtained with savinase have antioxidant activity through the SET and HAT mechanisms. The application of the obtained compounds for the development of functional foods or for food preservation should be considered.

Original language	English
Article number	415
Journal	Horticulturae
Volume	9
Issue number	4
DOIs	https://doi.org/10.3390/horticulturae9040415
State	Published - Apr 2023

Bibliographical note

Funding Information:
María Fernanda González Osuna acknowledges the Consejo Nacional de Ciencia y Tecnología (CONACyT, Mexico) for Ph.D. scholarship.

Funding Information:
This research was funded by Universidad de Sonora, grant number USO313007398.

Publisher Copyright:
© 2023 by the authors.

Keywords

antioxidant peptide fractions
chickpea protein hydrolysates
globulin
ultrasound pretreatment

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10.3390/horticulturae9040415

Cite this

González-Osuna, M. F., Torres-Arreola, W., Márquez-Ríos, E., Wong-Corral, F. J., Lugo-Cervantes, E., Rodríguez-Figueroa, J. C., García-Sánchez, G., Ezquerra-Brauer, J. M., Soto-Valdez, H., Castillo, A., & Del-Toro-Sánchez, C. L. (2023). Antioxidant Activity of Peptide Fractions from Chickpea Globulin Obtained by Pulsed Ultrasound Pretreatment. Horticulturae, 9(4), Article 415. https://doi.org/10.3390/horticulturae9040415

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abstract = "Protein hydrolysates and peptides can show biological activities, and pulsed ultrasound improves bioactivities. Among matrices from which protein hydrolysates can be obtain, chickpea is an excellent source. The objective of this research was to evaluate the effect of pulsed ultrasound on globulin concentrate to obtain chickpea hydrolysate (HGb) and peptide fractions and their bioactivity. Antioxidant activity by ABTS (2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt), FRAP (Ferric Reducing Antioxidant Power) and human erythrocyte assays was determined. The electrophoretic profile, amino acid profile, and antimicrobial activity of hydrolysates were also determined. Two hydrolysates had the highest antioxidant activity: HGb (91.44% ABTS inhibition, 73.04% hemolysis inhibition and 5185.57 µmol TE/g dried sample in FRAP assay) and HGb-20 (48.25% ABTS inhibition, 100% hemolysis inhibition and 2188.53 µmol TE/g dried sample in FRAP assay). Peptide fractions inhibited 100% of the hemolysis on human erythrocytes. The hydrolysates from chickpea proteins obtained with savinase have antioxidant activity through the SET and HAT mechanisms. The application of the obtained compounds for the development of functional foods or for food preservation should be considered.",

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González-Osuna, MF, Torres-Arreola, W, Márquez-Ríos, E, Wong-Corral, FJ, Lugo-Cervantes, E, Rodríguez-Figueroa, JC, García-Sánchez, G, Ezquerra-Brauer, JM, Soto-Valdez, H, Castillo, A & Del-Toro-Sánchez, CL 2023, 'Antioxidant Activity of Peptide Fractions from Chickpea Globulin Obtained by Pulsed Ultrasound Pretreatment', Horticulturae, vol. 9, no. 4, 415. https://doi.org/10.3390/horticulturae9040415

TY - JOUR

T1 - Antioxidant Activity of Peptide Fractions from Chickpea Globulin Obtained by Pulsed Ultrasound Pretreatment

AU - González-Osuna, María Fernanda

AU - Torres-Arreola, Wilfrido

AU - Márquez-Ríos, Enrique

AU - Wong-Corral, Francisco Javier

AU - Lugo-Cervantes, Eugenia

AU - Rodríguez-Figueroa, José Carlos

AU - García-Sánchez, Guillermina

AU - Ezquerra-Brauer, Josafat Marina

AU - Soto-Valdez, Herlinda

AU - Castillo, Alejandro

AU - Del-Toro-Sánchez, Carmen Lizette

PY - 2023/4

Y1 - 2023/4

N2 - Protein hydrolysates and peptides can show biological activities, and pulsed ultrasound improves bioactivities. Among matrices from which protein hydrolysates can be obtain, chickpea is an excellent source. The objective of this research was to evaluate the effect of pulsed ultrasound on globulin concentrate to obtain chickpea hydrolysate (HGb) and peptide fractions and their bioactivity. Antioxidant activity by ABTS (2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt), FRAP (Ferric Reducing Antioxidant Power) and human erythrocyte assays was determined. The electrophoretic profile, amino acid profile, and antimicrobial activity of hydrolysates were also determined. Two hydrolysates had the highest antioxidant activity: HGb (91.44% ABTS inhibition, 73.04% hemolysis inhibition and 5185.57 µmol TE/g dried sample in FRAP assay) and HGb-20 (48.25% ABTS inhibition, 100% hemolysis inhibition and 2188.53 µmol TE/g dried sample in FRAP assay). Peptide fractions inhibited 100% of the hemolysis on human erythrocytes. The hydrolysates from chickpea proteins obtained with savinase have antioxidant activity through the SET and HAT mechanisms. The application of the obtained compounds for the development of functional foods or for food preservation should be considered.

AB - Protein hydrolysates and peptides can show biological activities, and pulsed ultrasound improves bioactivities. Among matrices from which protein hydrolysates can be obtain, chickpea is an excellent source. The objective of this research was to evaluate the effect of pulsed ultrasound on globulin concentrate to obtain chickpea hydrolysate (HGb) and peptide fractions and their bioactivity. Antioxidant activity by ABTS (2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt), FRAP (Ferric Reducing Antioxidant Power) and human erythrocyte assays was determined. The electrophoretic profile, amino acid profile, and antimicrobial activity of hydrolysates were also determined. Two hydrolysates had the highest antioxidant activity: HGb (91.44% ABTS inhibition, 73.04% hemolysis inhibition and 5185.57 µmol TE/g dried sample in FRAP assay) and HGb-20 (48.25% ABTS inhibition, 100% hemolysis inhibition and 2188.53 µmol TE/g dried sample in FRAP assay). Peptide fractions inhibited 100% of the hemolysis on human erythrocytes. The hydrolysates from chickpea proteins obtained with savinase have antioxidant activity through the SET and HAT mechanisms. The application of the obtained compounds for the development of functional foods or for food preservation should be considered.

KW - antioxidant peptide fractions

KW - chickpea protein hydrolysates

KW - globulin

KW - ultrasound pretreatment

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U2 - 10.3390/horticulturae9040415

DO - 10.3390/horticulturae9040415

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AN - SCOPUS:85154538302

SN - 2311-7524

VL - 9

JO - Horticulturae

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M1 - 415

ER -

Antioxidant Activity of Peptide Fractions from Chickpea Globulin Obtained by Pulsed Ultrasound Pretreatment

Abstract

Bibliographical note

Keywords

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