TY - JOUR
T1 - Antioxidant, anti-inflammatory, and antiproliferative activities of Flourensia spp.
AU - Jasso de Rodríguez, Diana
AU - Torres-Moreno, Heriberto
AU - López-Romero, Julio César
AU - Vidal-Gutiérrez, Max
AU - Villarreal-Quintanilla, José Ángel
AU - Carrillo-Lomelí, Dennise Anahí
AU - Robles-Zepeda, Ramón Enrique
AU - Vilegas, Wagner
N1 - Funding Information:
Authors would like to thank to T.A. María Guadalupe Moreno Esquivel, T.A. Edith E. Chaires Colunga and C. Juan José Valenzuela Cabrera of the Phytochemistry Laboratory from UAAAN, for their invaluable assistance in the plant collection and extracts obtention.
Publisher Copyright:
© 2022
PY - 2023/1
Y1 - 2023/1
N2 - Lung cancer originates from inflammatory processes mediated by pro-inflammatory cytokines, such as tumor necrosis factor-alpha (TNF-α) or molecular markers, such as nitric oxide (NO). Flourensia cernua, F. retinophylla, and F. microphylla have been studied and shown to possess remarkable antioxidant and biological activities. Therefore, the present study aimed to determine the total phenolic content (TPC), antioxidant capacity, and chemical composition of F. cernua, F. retinophylla, and F. microphylla extracts, as well as its anti-inflammatory activities in vitro on RAW 264.7 macrophages cells. Further, the cytotoxic and antiproliferative activities in vitro were assessed using A549 lung cancer cells and ARPE-19 cells. The TPC were assessed by the Folin-Ciocalteu method; antioxidant activity was assessed using the ABTS, DPPH, and FRAP techniques, and the chemical composition was determined by ESI-IT-MSn. The cytotoxic and antiproliferative properties were measured using the MTT assay. The anti-inflammatory activity was determined by evaluating the inhibition of NO and TNF-α production. The results showed that F. microphylla presented the highest TPC value of 284.83 mg GAE/g DW, and antioxidant activity with values of 20.4 μg TE/mL, 6.7 μg TE/mL, 3122.9 μM Fe(II)/g DW for DPPH, ABTS, and FRAP assays, respectively. A total of nine, five, and nine chemical compounds were identified in F. cernua, F. retinophylla, and F. microphylla, respectively. Moreover, the three extracts evaluated presented antiproliferative effect on A549 cells, and F. cernua demonstrated the best anti-inflammatory activity. Therefore, these extracts could be used as natural anti-inflammatory agents to control lung cancer.
AB - Lung cancer originates from inflammatory processes mediated by pro-inflammatory cytokines, such as tumor necrosis factor-alpha (TNF-α) or molecular markers, such as nitric oxide (NO). Flourensia cernua, F. retinophylla, and F. microphylla have been studied and shown to possess remarkable antioxidant and biological activities. Therefore, the present study aimed to determine the total phenolic content (TPC), antioxidant capacity, and chemical composition of F. cernua, F. retinophylla, and F. microphylla extracts, as well as its anti-inflammatory activities in vitro on RAW 264.7 macrophages cells. Further, the cytotoxic and antiproliferative activities in vitro were assessed using A549 lung cancer cells and ARPE-19 cells. The TPC were assessed by the Folin-Ciocalteu method; antioxidant activity was assessed using the ABTS, DPPH, and FRAP techniques, and the chemical composition was determined by ESI-IT-MSn. The cytotoxic and antiproliferative properties were measured using the MTT assay. The anti-inflammatory activity was determined by evaluating the inhibition of NO and TNF-α production. The results showed that F. microphylla presented the highest TPC value of 284.83 mg GAE/g DW, and antioxidant activity with values of 20.4 μg TE/mL, 6.7 μg TE/mL, 3122.9 μM Fe(II)/g DW for DPPH, ABTS, and FRAP assays, respectively. A total of nine, five, and nine chemical compounds were identified in F. cernua, F. retinophylla, and F. microphylla, respectively. Moreover, the three extracts evaluated presented antiproliferative effect on A549 cells, and F. cernua demonstrated the best anti-inflammatory activity. Therefore, these extracts could be used as natural anti-inflammatory agents to control lung cancer.
KW - Anti-inflammatory
KW - Flourensia spp.
KW - Lung cancer
KW - Nitric oxide
KW - Polyphenolic compounds
KW - Tumor necrosis factor-alpha
UR - http://www.scopus.com/inward/record.url?scp=85142691350&partnerID=8YFLogxK
U2 - 10.1016/j.bcab.2022.102552
DO - 10.1016/j.bcab.2022.102552
M3 - Artículo
AN - SCOPUS:85142691350
SN - 1878-8181
VL - 47
JO - Biocatalysis and Agricultural Biotechnology
JF - Biocatalysis and Agricultural Biotechnology
M1 - 102552
ER -