Antiproliferative activity of spinasterol isolated of Stegnosperma halimifolium (Benth, 1844)

Salvador Enrique Meneses-Sagrero, Moisés Navarro-Navarro, Eduardo Ruiz-Bustos, Carmen Lizette Del-Toro-Sánchez, Manuel Jiménez-Estrada, Ramón Enrique Robles-Zepeda*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Cancer is the major cause of death in the world, representing a significant public health problem. Plants have been shown as a great source of secondary metabolites with anticancer activity. The aim of this work was evaluated the antiproliferative activity of the methanolic extracts, chemical fractions and the compound spinasterol isolated of medicinal plant Stegnosperma halimifolium. The methanolic extracts of stem, leaf and stem/leaf was obtained by maceration. The methanolic extract of stem was purified by successive extractions with solvents as n-hexane, ethyl acetate and ethanol. The n-hexane fraction was separated by column chromatographic and monitored by thin layer chromatographic. The compound spinasterol was characterized by 1H NMR, 13C NMR and Mass Spectrometry. Methanolic extracts, chemical, chromatographic fractions and spinasterol was evaluated against RAW 264.7, M12.C3.F6, PC-3, LS-180, A549 and HeLa cancer cell lines by the standardized method MTT for determinate the antiproliferative activity. Methanolic extract of stem shown the better antiproliferative activity against the murine macrophage cancer cell line RAW 264.7. n-Hexane chemical fraction shown antiproliferative activity against human alveolar cancer cell line A549 and RAW 264.7. Was isolated and characterized a compound by NMR 1H and 13C, revealing the presence of sterol spinasterol. Spinasterol shown to have antiproliferative activity against cervical cancer cell line HeLa and RAW 264.7, indicating that spinasterol can be a responsible compound of antiproliferative activity found in the methanolic extract of Stegnosperma halimifolium.

Original languageEnglish
Pages (from-to)1137-1143
Number of pages7
JournalSaudi Pharmaceutical Journal
Volume25
Issue number8
DOIs
StatePublished - Dec 2017

Bibliographical note

Publisher Copyright:
© 2017 The Authors

Keywords

  • Antiproliferative activity
  • Spinasterol
  • Stegnosperma halimifolium
  • Stegnospermaceae

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