Antiproliferative activity of standardized phytopreparations from Ibervillea sonorae (S. Watson) Greene

Max Vidal-Gutiérrez, Heriberto Torres-Moreno, Salomón Hernández-Gutiérrez, Carlos Velazquez, Ramón E. Robles-Zepeda, Wagner Vilegas

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Ibervillea sonorae (Cucurbitaceae) is a medicinal plant utilized in Northwest Mexico against Diabetes and cancer. This natural product is taken orally, its presentation is capsules containing the plant's dried and powdered caudices. There is no regulation or standardized dosage that allows reproducibility of its pharmacological effects. Cucurbitacins are the main group of compounds found in I. sonorae and are known for their antiproliferative activity in cancer cells. Cucurbitacin IIb (CIIb), one of the compounds present in I. sonorae, has demonstrated in experimental models with HeLa cervical cancer cells an apoptotic and anti-tumoral activity. The objective of this study is to obtain and standardize two phytopreparations of I. sonorae based on their CIIb content, evaluate their antiproliferative activity in cancer cell lines, and compare the results with those obtained with CIIb; expecting to find phytopreparations with anti-cancer potential. APCI-IT-MSn is utilized for the identification of cucurbitacins, FT-ICR-MS/MS for the quantification of CIIb, and the MTT assay for the evaluation of the antiproliferative activity. The CIIb content was 0.67% for Fito-Ison-EtOH and 1.84% for Fito-Ison-EtOAc. In both phytopreparations, six cucurbitacins have been identified, and a seventh one not previously identified. Phytopreparations were more effective against HeLa, with IC50 of 30.0 and 18.6 µg/mL for Fito-Ison-EtOH and Fito-Ison-EtOAc, respectively. This effect is lower than observed on CIIb in HeLa (5.8 µg/mL). There are no significant differences (p>0.05) in the antiproliferative activity between Fito-Ison-EtOAc and CIIb in A549, LS180, and MDA-MB-231 cells. Phytopreparations of I. sonorae have potential for the development of anti-cancer products.

Original languageEnglish
Article number108824
Pages (from-to)108824
Early online date13 Mar 2021
StatePublished - 1 May 2021

Bibliographical note

Funding Information:
This study was financed in part by the Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - Brasil (CAPES) - Finance Code 001. This work was partially financed by the National Council for Science and Technology of Mexico (CONACYT, 83462).

Publisher Copyright:
© 2021


  • Antiproliferative
  • Cucurbitacin IIb
  • Cucurbitacins
  • Ibervillea sonorae
  • Phytopreparation
  • Triterpenes


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