TY - JOUR
T1 - Antiproliferative and apoptosis induction of cucurbitacin-type triterpenes from Ibervillea sonorae
AU - Torres-Moreno, H.
AU - Velázquez, C. A.
AU - Garibay-Escobar, A.
AU - Curini, Massimo
AU - Marcotullio, M. C.
AU - Robles-Zepeda, R. E.
PY - 2015/12/23
Y1 - 2015/12/23
N2 - © 2015 Elsevier B.V. Two cucurbitacin-type triterpenes kinoin A (1) and kinoin B diglucoside (2) were isolated and characterized by chromatographic and spectroscopic techniques from the root of Ibervillea sonorae. By MTT assay their antiproliferative activity was evaluated and only compound 1 was able to inhibit the growth of HeLa, M12AK.C3F6, A549 and RAW 264.7 cancer cell lines with a IC50 of 18.0, 31.4, 40.2, and 61.5μM, respectively. L-929 cell line used as a control was more resistant to compound 1 at IC50 values greater than 100μM. Apoptosis induction was quantified for a double staining by Annexin V/propidium iodide using flow cytometry, and it was observed that treatment of HeLa cells with compound 1 cause cell death by apoptosis.
AB - © 2015 Elsevier B.V. Two cucurbitacin-type triterpenes kinoin A (1) and kinoin B diglucoside (2) were isolated and characterized by chromatographic and spectroscopic techniques from the root of Ibervillea sonorae. By MTT assay their antiproliferative activity was evaluated and only compound 1 was able to inhibit the growth of HeLa, M12AK.C3F6, A549 and RAW 264.7 cancer cell lines with a IC50 of 18.0, 31.4, 40.2, and 61.5μM, respectively. L-929 cell line used as a control was more resistant to compound 1 at IC50 values greater than 100μM. Apoptosis induction was quantified for a double staining by Annexin V/propidium iodide using flow cytometry, and it was observed that treatment of HeLa cells with compound 1 cause cell death by apoptosis.
UR - http://dx.doi.org/10.1016/j.indcrop.2015.09.055
U2 - 10.1016/j.indcrop.2015.09.055
DO - 10.1016/j.indcrop.2015.09.055
M3 - Article
SP - 895
EP - 900
JO - Industrial Crops and Products
JF - Industrial Crops and Products
SN - 0926-6690
ER -