Antiproliferative Potential of Olneya tesota PF2 Lectin in Human Acute Monocytic Leukemia Cells

Diana Villegas-Coronado, Jesús Adriana Soto-Guzman, Juan Manuel Martínez-Soto, Nayelli Guadalupe Teran-Saavedra, Ana Maria Guzman-Partida, Luz Vazquez-Moreno, Ana Gloria Villalba-Villalba, Amir Maldonado*, Irlanda Lagarda-Diaz*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Acute monocytic leukemia is a type of myeloid leukemia that develops in monocytes. The current clinical therapies for leukemia are unsatisfactory due to their side effects and nonspecificity toward target cells. Some lectins display antitumor activity and may specifically recognize cancer cells by binding to carbohydrate structures on their surface. Therefore, this study evaluated the response of the human monocytic leukemia cell lines THP-1 to the Olneya tesota PF2 lectin. The induction of apoptosis and reactive oxygen species production in PF2-treated cells was evaluated by flow cytometry, and the lectin-THP-1 cell interaction and mitochondrial membrane potential were evaluated by confocal fluorescence microscopy. PF2 genotoxicity was evaluated by DNA fragmentation analysis via gel electrophoresis. The results showed that PF2 binds to THP-1 cells, triggers apoptosis and DNA degradation, changes the mitochondrial membrane potential, and increases reactive oxygen species levels in PF2-treated THP-1 cells. These results suggest the potential use of PF2 for developing alternative anticancer treatments with enhanced specificity.

Original languageEnglish
Article numbere202300051
JournalChemistry and Biodiversity
Volume20
Issue number7
DOIs
StatePublished - Jul 2023

Bibliographical note

Publisher Copyright:
© 2023 Wiley-VHCA AG, Zurich, Switzerland.

Keywords

  • PF2 lectin
  • THP-1 cells
  • acute myeloid leukemia
  • anticancer
  • apoptosis
  • cytotoxic

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