Antiviral, antioxidant, and antihemolytic effect of annona muricata L. Leaves extracts

Ana Paola Balderrama-Carmona, Norma Patricia Silva-Beltrán*, Juan Carlos Gálvez-Ruiz, Saúl Ruíz-Cruz, Cristóbal Chaidez-Quiroz, Edgar Felipe Morán-Palacio

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Annona muricata L. is a tropical tree that is used in traditional medicine around the world. The high content of flavonoid, alkaloid, acetogenin, phenolic and lipophilic compounds of this tropical tree forms the basis of its traditional medical uses. Our objective was to study soursop leaf extracts to support their use as antiviral therapies and investigate their protective effects against oxidative damage. The aqueous extract (AE) and acidified ethanolic extract (AEE) of soursop leaves were characterized by ultra performance liquid chromatography (UPLC), and their effects on human erythrocytes and in vitro antioxidant capacity, as evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assays, were investigated. The antiviral effects were evaluated using a bacteriophage surrogate. AEE showed the highest phenolic content, with rutin as the predominant compound. This extract showed higher values in the DPPH and ABTS assays, with 23.61 ± 0.42 and 24.91 ± 0.16 mmol of Trolox equivalent per gram, respectively. Inhibition of hemolysis was 34% and 51% for AE and AEE, respectively. AEE was selected for the antiviral study because of its higher antioxidant activity. The viral reduction ranged from 5–6 log10 plaque-forming units/volume (PFU) at contact times of 15–360 min. Soursop leaves have a positive effect on reducing oxidative stress in human erythrocytes and viral infections.

Original languageEnglish
Article number1650
Pages (from-to)1-11
Number of pages11
JournalPlants
Volume9
Issue number12
DOIs
StatePublished - Dec 2020

Bibliographical note

Funding Information:
Funding: This work was supported by Fondos Mixtos Consejo Nacional de Ciencia y Tecnología and CONACyT.

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.

Keywords

  • Antioxidant capacity
  • Antiviral
  • Bacteriophages
  • Rutin
  • Soursop
  • UPLC

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