Assessment of the Route of Exposure to Ovalbumin and Cow’s Milk Proteins on the Induction of IgE Responses in BALB/c Mice

Feliznando Isidro Cárdenas-Torres, Francisco Cabrera-Chávez, Aldo Alejandro Arvizu-Flores, Lilian Karem Flores-Mendoza, Veronica Lopez-Teros, Humberto Astiazaran-Garcia, Martina Hilda Gracia-Valenzuela, Oscar Gerardo Figueroa-Salcido, Jesús Gilberto Arámburo-Gálvez*, Noé Ontiveros*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


BALB/c mice can be orally sensitized to food proteins under acid suppressive medication, mimicking human exposure and triggering a human-like allergic immune response. However, the reproducibility of such an oral food allergy model remains questionable. Our aim was to evaluate the IgE responses triggered against ovalbumin (OVA) and cow’s milk proteins (CMP) after intragastric (IG), either under gastric-acid suppression or not, or intraperitoneal (IP) sensitization in BALB/c mice. OVA (0.2 mg) and different concentrations of CMP were administered with/without the antacid sucralfate by the IG route. For IP sensitization, OVA or CMP (0.5 mg) were administered. ELISA was used to evaluate IgE responses. The IP sensitization protocols triggered more robust and consistent anti-OVA or anti-CMP IgE responses than the intragastric ones (with/without sucralfate) (p < 0.05). 2.7% (1/36), and 5.5% (3/54) of the mice that underwent the sucralfate-assisted IG protocol triggered IgE responses against OVA or CMP, respectively. All the mice were administered OVA or CMP via IP triggered detectable IgE responses. The IP sensitization model is more reliable than the IG one for evaluating the intrinsic sensitizing and/or allergenic potential of food proteins, even if IG immunizations are carried out under gastric-acid suppression.

Original languageEnglish
Article number542
Issue number4
StatePublished - Apr 2022

Bibliographical note

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© 2022 by the authors. Licensee MDPI, Basel, Switzerland.


  • BALB/c mice
  • cow’s milk proteins
  • food allergy
  • intragastric sensitization
  • intraperitoneal sensitization
  • ovalbumin


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