Bacterial incidence and drug resistance from pathogens recovered from blood, cerebrospinal and pleural fluids in 2019–2020. Results of the Invifar network

Elvira Garza-González, Adrian Camacho-Ortiz, Alfredo Ponce-De-Leon, Edgar Ortiz-Brizuela, Luis Esaú López-Jácome, Claudia Colin, Fabian Rojas-Larios, Oscar A. Newton-Sánchez, Gabriela Echaniz-Aviles, Maria Noemi Carnalla-Barajas, Araceli Soto, Paola Bocanegra-Ibarias, Ana María del Rocío Hernández-Dueñas, María del Consuelo Velázquez-Acosta, Laura Karina Avilés-Benítez, Juan Pablo Mena-Ramirez, Daniel Romero, Isela Mora-Jiménez, Margarita Alcaraz-Espejel, José Manuel Feliciano-GuzmánMaribel López-García, Patricia Rodriguez-Zulueta, María Angelina Quevedo-Ramos, Cecilia Padilla-Ibarra, Carlos Antonio Couoh-May, Maria Carolina Rivera-Ferreira, Cecilia Teresita Morales-De-la-Peña, Hector Zubiate, Raúl Peralta-Catalán, Carlos Miguel Cetina-Umaña, Joaquin Rincón-Zuno, Maria Lucia Perez-Ricardez, Iris Yazmin Hernández-Cordova, Eduardo López-Gutiérrez, Mariana Gil, Efren Aguirre-Burciaga, Guadalupe Soledad Huirache-Villalobos, Scarlett Munoz, Nicolás Rogelio Eric Barlandas-Rendón, Enrique Bolado-Martinez, Luis Javier Quintanilla-Cazares, Abraham C. Gómez-Choel, Laura Lopez, Juan Carlos Tinoco, Rosa Areli Martínez-Gamboa, Alejandro Molina, Samuel Pavel Escalante-Armenta, Lizbeth Duarte, Luis Alberto Ruiz-Gamboa, Dulce Isabel Cobos-Canul, Dulce López, Irma Elena Barroso-Herrera-y-Cairo, Eduardo Rodriguez-Noriega, Rayo Morfin-Otero*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Background. Antimicrobial resistance is a global concern. Analysis of sterile fluids is essential because microorganisms are defined as significant in most cases. Blood, cerebrospinal, and pleural fluids are frequently received in the microbiology lab because they are associated with considerable rates of morbi-mortality. Knowledge of epidemiology in these samples is needed to choose proper empirical treatments due to the importance of reducing selection pressure. Methods. We used retrospective laboratory data of blood, CSF, and pleural fluid collected from patients in Mexico between 2019 and 2020. Each laboratory identified the strains and tested susceptibility using its routine methods. For Streptococcus pneumoniae, a comparative analysis was performed with data from the broth microdilution method. Results. Forty-five centers participated in the study, with 30,746 clinical isolates from blood, 2,429 from pleural fluid, and 2,275 from CSF. For blood and CSF, Staphylococcus epidermidis was the most frequent. For blood, among gram negatives, the most frequent was Escherichia coli. Among Enterobacterales, 9.8% of K. pneumoniae were carbapenem-resistant. For S. pneumoniae, similar resistance percentages were observed for levofloxacin, cefotaxime, and vancomycin. For CSF, the most frequent gram-negative was E. coli. In Acinetobacter baumannii, carbapenem resistance was 71.4%. The most frequent species detected for pleural fluid was E. coli; in A. baumannii, carbapenem resistance was 96.3%. Conclusion. Gram-negative bacteria, with E. coli most prevalent, are frequently recovered from CSF, blood, and pleural fluid. In S. pneumoniae, the routine, conventional methods showed good agreement in detecting resistance percentages for erythromycin, levofloxacin, and vancomycin.

Original languageEnglish
Article numbere14411
JournalPeerJ
Volume11
DOIs
StatePublished - Jan 2023

Bibliographical note

Publisher Copyright:
Copyright 2023 Garza-González et al.

Keywords

  • Blood stream infection
  • Cerebrospinal infection
  • Drug resistance
  • Escherichia coli
  • INVIFAR

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