Bacterial Morphotypes as Important Trait for Uropathogenic E. coli Diagnostic; a Virulence-Phenotype-Phylogeny Study

Manuel G. Ballesteros-Monrreal; Margarita M.P. Arenas-Hernández; Edwin Barrios-Villa; Josué Juárez; Maritza Lizeth Álvarez-Ainza; Pablo Taboada; Rafael De la Rosa-López; Enrique Bolado Martínez; Dora Edith Valencia Rivera

doi:10.3390/microorganisms9112381

Bacterial Morphotypes as Important Trait for Uropathogenic E. coli Diagnostic; a Virulence-Phenotype-Phylogeny Study

Manuel G. Ballesteros-Monrreal, Margarita M.P. Arenas-Hernández, Edwin Barrios-Villa, Josué Juárez, Maritza Lizeth Álvarez-Ainza, Pablo Taboada, Rafael De la Rosa-López, Enrique Bolado Martínez, Dora Edith Valencia Rivera^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Urinary tract infections (UTIs) belong to the most common pathologies in Mexico and are mainly caused by Uropathogenic Escherichia coli (UPEC). UPEC possesses a wide diversity of virulence factors that allow it to carry out its pathogenesis mechanism in the urinary tract (UT). The development of morphotypes in UT represents an important feature of UPEC because it is associated with complications in diagnosis of UTI. The aim of this study was to determine the presence of bacterial morphotypes, virulence genes, virulence phenotypes, antibiotic resistant, and phylogenetic groups in clinical isolates of UPEC obtained from women in Sonora, Mexico. Forty UPEC isolates were obtained, and urine morphotypes were observed in 65% of the urine samples from where E. coli was isolated. Phylogenetic group B2 was the most prevalent. The most frequent virulence genes were fimH (100%), fliCD (90%), and sfaD/focC (72%). Biofilm formation (100%) and motility (98%) were the most prevalent phenotypes. Clinical isolates showed high resistance to aminoglycosides and β-lactams antibiotics. These data suggest that the search for morphotypes in urine sediment must be incorporated in the urinalysis procedure and also that clinical isolates of UPEC in this study can cause upper, lower, and recurrent UTI.

Translated title of the contribution	Morfotipos Bacterianos como Característica Importante para el Diagnóstico de E. coli Uropatógena; Un Estudio de Virulencia, Fenotipos y Filogenia
Original language	English
Article number	2381
Pages (from-to)	1-21
Number of pages	21
Journal	Microorganisms
Volume	9
Issue number	2381
DOIs	https://doi.org/10.3390/microorganisms9112381
State	Published - 18 Nov 2021

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Ballesteros-Monrreal, M. G., Arenas-Hernández, M. M. P., Barrios-Villa, E., Juárez, J., Álvarez-Ainza, M. L., Taboada, P., De la Rosa-López, R., Bolado Martínez, E., & Valencia Rivera, D. E. (2021). Bacterial Morphotypes as Important Trait for Uropathogenic E. coli Diagnostic; a Virulence-Phenotype-Phylogeny Study. Microorganisms, 9(2381), 1-21. Article 2381. https://doi.org/10.3390/microorganisms9112381

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abstract = "Urinary tract infections (UTIs) belong to the most common pathologies in Mexico and are mainly caused by Uropathogenic Escherichia coli (UPEC). UPEC possesses a wide diversity of virulence factors that allow it to carry out its pathogenesis mechanism in the urinary tract (UT). The development of morphotypes in UT represents an important feature of UPEC because it is associated with complications in diagnosis of UTI. The aim of this study was to determine the presence of bacterial morphotypes, virulence genes, virulence phenotypes, antibiotic resistant, and phylogenetic groups in clinical isolates of UPEC obtained from women in Sonora, Mexico. Forty UPEC isolates were obtained, and urine morphotypes were observed in 65% of the urine samples from where E. coli was isolated. Phylogenetic group B2 was the most prevalent. The most frequent virulence genes were fimH (100%), fliCD (90%), and sfaD/focC (72%). Biofilm formation (100%) and motility (98%) were the most prevalent phenotypes. Clinical isolates showed high resistance to aminoglycosides and β-lactams antibiotics. These data suggest that the search for morphotypes in urine sediment must be incorporated in the urinalysis procedure and also that clinical isolates of UPEC in this study can cause upper, lower, and recurrent UTI.",

keywords = "Urinary tract infection, UPEC morphotypes, UPEC virulence",

author = "Ballesteros-Monrreal, {Manuel G.} and Arenas-Hern{\'a}ndez, {Margarita M.P.} and Edwin Barrios-Villa and Josu{\'e} Ju{\'a}rez and {\'A}lvarez-Ainza, {Maritza Lizeth} and Pablo Taboada and {De la Rosa-L{\'o}pez}, Rafael and {Bolado Mart{\'i}nez}, Enrique and {Valencia Rivera}, {Dora Edith}",

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Ballesteros-Monrreal, MG, Arenas-Hernández, MMP, Barrios-Villa, E , Juárez, J, Álvarez-Ainza, ML, Taboada, P, De la Rosa-López, R, Bolado Martínez, E & Valencia Rivera, DE 2021, 'Bacterial Morphotypes as Important Trait for Uropathogenic E. coli Diagnostic; a Virulence-Phenotype-Phylogeny Study', Microorganisms, vol. 9, no. 2381, 2381, pp. 1-21. https://doi.org/10.3390/microorganisms9112381

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T1 - Bacterial Morphotypes as Important Trait for Uropathogenic E. coli Diagnostic; a Virulence-Phenotype-Phylogeny Study

AU - Ballesteros-Monrreal, Manuel G.

AU - Arenas-Hernández, Margarita M.P.

AU - Barrios-Villa, Edwin

AU - Juárez, Josué

AU - Álvarez-Ainza, Maritza Lizeth

AU - Taboada, Pablo

AU - De la Rosa-López, Rafael

AU - Bolado Martínez, Enrique

AU - Valencia Rivera, Dora Edith

PY - 2021/11/18

Y1 - 2021/11/18

N2 - Urinary tract infections (UTIs) belong to the most common pathologies in Mexico and are mainly caused by Uropathogenic Escherichia coli (UPEC). UPEC possesses a wide diversity of virulence factors that allow it to carry out its pathogenesis mechanism in the urinary tract (UT). The development of morphotypes in UT represents an important feature of UPEC because it is associated with complications in diagnosis of UTI. The aim of this study was to determine the presence of bacterial morphotypes, virulence genes, virulence phenotypes, antibiotic resistant, and phylogenetic groups in clinical isolates of UPEC obtained from women in Sonora, Mexico. Forty UPEC isolates were obtained, and urine morphotypes were observed in 65% of the urine samples from where E. coli was isolated. Phylogenetic group B2 was the most prevalent. The most frequent virulence genes were fimH (100%), fliCD (90%), and sfaD/focC (72%). Biofilm formation (100%) and motility (98%) were the most prevalent phenotypes. Clinical isolates showed high resistance to aminoglycosides and β-lactams antibiotics. These data suggest that the search for morphotypes in urine sediment must be incorporated in the urinalysis procedure and also that clinical isolates of UPEC in this study can cause upper, lower, and recurrent UTI.

AB - Urinary tract infections (UTIs) belong to the most common pathologies in Mexico and are mainly caused by Uropathogenic Escherichia coli (UPEC). UPEC possesses a wide diversity of virulence factors that allow it to carry out its pathogenesis mechanism in the urinary tract (UT). The development of morphotypes in UT represents an important feature of UPEC because it is associated with complications in diagnosis of UTI. The aim of this study was to determine the presence of bacterial morphotypes, virulence genes, virulence phenotypes, antibiotic resistant, and phylogenetic groups in clinical isolates of UPEC obtained from women in Sonora, Mexico. Forty UPEC isolates were obtained, and urine morphotypes were observed in 65% of the urine samples from where E. coli was isolated. Phylogenetic group B2 was the most prevalent. The most frequent virulence genes were fimH (100%), fliCD (90%), and sfaD/focC (72%). Biofilm formation (100%) and motility (98%) were the most prevalent phenotypes. Clinical isolates showed high resistance to aminoglycosides and β-lactams antibiotics. These data suggest that the search for morphotypes in urine sediment must be incorporated in the urinalysis procedure and also that clinical isolates of UPEC in this study can cause upper, lower, and recurrent UTI.

KW - Urinary tract infection

KW - UPEC morphotypes

KW - UPEC virulence

U2 - 10.3390/microorganisms9112381

DO - 10.3390/microorganisms9112381

M3 - Artículo

C2 - 34835506

SN - 2076-2607

VL - 9

SP - 1

EP - 21

JO - Microorganisms

JF - Microorganisms

IS - 2381

M1 - 2381

ER -