Abstract
The white spot syndrome virus (WSSV) is the most lethal virus that infect shrimps, generating economic losses for aquaculture industry. Osmotic stress compromises the immune response in WSSV-infected shrimp. Betaine aldehyde dehydrogenase (BADH E.C. 1.2.1.8) is an enzyme that participates in the regulation of osmotic stress, and that is up-regulated in shrimp exposed to changes in salinity concentration. However, different gene expression studies in shrimp during WSSV infection focused in changes on the mechanisms of energy production and cellular defense genes, without considering genes sensitive to osmotic variation. In this work, we evaluate the mRNA levels and activity of the BADH enzyme, as well as glycine betaine (GB) accumulation, during WSSV infection in white shrimp. Shrimps were inoculated with WSSV and samples from the hepatopancreas were collected at 24 and 48 h post-infection. Our results demonstrate that WSSV infection increases the expression of BADH 1.5-fold at 48h and the enzyme activity increase 4.28- and 4.59-fold at 24 and 48 h, respectively. Also, higher accumulation of GB was observed during WSSV infection. These results suggest that BADH could participate in the cellular response during WSSV infection in white shrimp.
| Original language | English |
|---|---|
| Pages (from-to) | 113-119 |
| Number of pages | 7 |
| Journal | Invertebrate Survival Journal |
| Volume | 16 |
| Issue number | 1 |
| State | Published - 22 Jan 2019 |
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Keywords
- Betaine aldehyde dehydrogenase
- Glycine betaine
- MRNA expression
- Shrimp
- White spot syndrome virus
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Betaine aldehyde dehydrogenase is regulated during wssv infection in white shrimp. / Rosas-Rodríguez, J. A.; Soñanez-Organis, J. G.; Miranda-Cruz, M. M.; Poom-Llamas, J. J.; Stephens-Camacho, N. A.; Adan-Bante, N. P.
In: Invertebrate Survival Journal, Vol. 16, No. 1, 22.01.2019, p. 113-119.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Betaine aldehyde dehydrogenase is regulated during wssv infection in white shrimp
AU - Rosas-Rodríguez, J. A.
AU - Soñanez-Organis, J. G.
AU - Miranda-Cruz, M. M.
AU - Poom-Llamas, J. J.
AU - Stephens-Camacho, N. A.
AU - Adan-Bante, N. P.
PY - 2019/1/22
Y1 - 2019/1/22
N2 - The white spot syndrome virus (WSSV) is the most lethal virus that infect shrimps, generating economic losses for aquaculture industry. Osmotic stress compromises the immune response in WSSV-infected shrimp. Betaine aldehyde dehydrogenase (BADH E.C. 1.2.1.8) is an enzyme that participates in the regulation of osmotic stress, and that is up-regulated in shrimp exposed to changes in salinity concentration. However, different gene expression studies in shrimp during WSSV infection focused in changes on the mechanisms of energy production and cellular defense genes, without considering genes sensitive to osmotic variation. In this work, we evaluate the mRNA levels and activity of the BADH enzyme, as well as glycine betaine (GB) accumulation, during WSSV infection in white shrimp. Shrimps were inoculated with WSSV and samples from the hepatopancreas were collected at 24 and 48 h post-infection. Our results demonstrate that WSSV infection increases the expression of BADH 1.5-fold at 48h and the enzyme activity increase 4.28- and 4.59-fold at 24 and 48 h, respectively. Also, higher accumulation of GB was observed during WSSV infection. These results suggest that BADH could participate in the cellular response during WSSV infection in white shrimp.
AB - The white spot syndrome virus (WSSV) is the most lethal virus that infect shrimps, generating economic losses for aquaculture industry. Osmotic stress compromises the immune response in WSSV-infected shrimp. Betaine aldehyde dehydrogenase (BADH E.C. 1.2.1.8) is an enzyme that participates in the regulation of osmotic stress, and that is up-regulated in shrimp exposed to changes in salinity concentration. However, different gene expression studies in shrimp during WSSV infection focused in changes on the mechanisms of energy production and cellular defense genes, without considering genes sensitive to osmotic variation. In this work, we evaluate the mRNA levels and activity of the BADH enzyme, as well as glycine betaine (GB) accumulation, during WSSV infection in white shrimp. Shrimps were inoculated with WSSV and samples from the hepatopancreas were collected at 24 and 48 h post-infection. Our results demonstrate that WSSV infection increases the expression of BADH 1.5-fold at 48h and the enzyme activity increase 4.28- and 4.59-fold at 24 and 48 h, respectively. Also, higher accumulation of GB was observed during WSSV infection. These results suggest that BADH could participate in the cellular response during WSSV infection in white shrimp.
KW - Betaine aldehyde dehydrogenase
KW - Glycine betaine
KW - MRNA expression
KW - Shrimp
KW - White spot syndrome virus
UR - http://www.scopus.com/inward/record.url?scp=85071326492&partnerID=8YFLogxK
M3 - Artículo
VL - 16
SP - 113
EP - 119
JO - Invertebrate Survival Journal
JF - Invertebrate Survival Journal
SN - 1824-307X
IS - 1
ER -