TY - JOUR
T1 - Binding of PF2 lectin from Olneya tesota to gut proteins of Zabrotes subfasciatus larvae associated with the insecticidal mechanism
AU - Lagarda-Diaz, Irlanda
AU - Robles-Burgeño, M. Refugio
AU - Guzman-Partida, Ana M.
AU - Geiser, Dawn
AU - Winzerling, Joy
AU - Vazquez-Moreno, Luz
PY - 2012/3/7
Y1 - 2012/3/7
N2 - Zabrotes subfasciatus (Boheman) is the main pest of common beans (Phaselous vulgaris). Wild legume seeds from Olneya tesota contain a lectin, PF2, that shows insecticidal activities against this insect. The binding of PF2 to midgut glycoproteins of 20-day-old larvae was evaluated using PF2 affinity chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the proteins retained on the gel revealed several putative glycoproteins, ranging in mass from 17 to 97 kDa. Subsequent protein digestion and analysis by liquid chromatography-tandem mass spectrometry (LC-MS/ MS) provided amino acid fragments that identified an α-tubulin, cytochrome c oxidase subunit I, an odorant receptor, and a lysozyme from available insect sequence databases. The potential of these proteins to serve as part of the mechanisms involved in the insecticidal activity of PF2 to Z. subfasciatus is discussed.
AB - Zabrotes subfasciatus (Boheman) is the main pest of common beans (Phaselous vulgaris). Wild legume seeds from Olneya tesota contain a lectin, PF2, that shows insecticidal activities against this insect. The binding of PF2 to midgut glycoproteins of 20-day-old larvae was evaluated using PF2 affinity chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the proteins retained on the gel revealed several putative glycoproteins, ranging in mass from 17 to 97 kDa. Subsequent protein digestion and analysis by liquid chromatography-tandem mass spectrometry (LC-MS/ MS) provided amino acid fragments that identified an α-tubulin, cytochrome c oxidase subunit I, an odorant receptor, and a lysozyme from available insect sequence databases. The potential of these proteins to serve as part of the mechanisms involved in the insecticidal activity of PF2 to Z. subfasciatus is discussed.
KW - Affinity chromatography
KW - Insect midgut glycoproteins
KW - Olneya tesota
KW - PF2 lectin
KW - Wild legume
KW - Zabrotes subfasciatus
UR - http://www.scopus.com/inward/record.url?scp=84859738638&partnerID=8YFLogxK
U2 - 10.1021/jf2045872
DO - 10.1021/jf2045872
M3 - Artículo
SN - 0021-8561
VL - 60
SP - 2398
EP - 2402
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 9
ER -