Bioactive compounds from Octopus vulgaris ink extracts exerted anti-proliferative and anti-inflammatory effects in vitro

Martín S. Hernández-Zazueta, Iván Luzardo-Ocampo, Joel S. García-Romo, Luis Noguera-Artiaga, Ángel A. Carbonell-Barrachina, Pablo Taboada-Antelo, Rocío Campos-Vega, Ema Carina Rosas-Burgos, María G. Burboa-Zazueta, Josafat M. Ezquerra-Brauer, Armando Burgos-Hernández*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Underutilized marine food products such as cephalopods’ ink could be sources of bioactive compounds providing health benefits. This study aimed to assess the anti-proliferative and anti-inflammatory effects from Octopus vulgaris ink extracts (hexane-, ethyl acetate-, dichloromethane- (DM), and water extracts) using human colorectal (HT-29/HCT116) and breast (MDA-MB-231) cancer cells, and LPS-challenged murine RAW 264.7 cells. Except by ethyl-acetate, all of the extracts exhibited anti-proliferative effects without being cytotoxic to ARPE-19 and RAW 264.7 cells. Among DM fractions (F1/F2/F3), DM-F2 showed the highest anti-proliferative effect (LC50 = 52.64 μg/mL), inducing pro-apoptotic morphological disruptions in HCT116 cells. On RAW 264.7 cells, DM-F2 displayed the lowest nitrites reduction and up-regulation of key-cytokines from the JAK-STAT, PI3K-Akt, and IL-17 pathways. Compared to control, DM-F2 increased IL-4 and decreased NF-κB fluorometric expression in peripheral blood mononuclear cells (PBMCs). Metabolomic analysis of DM-F2 highlighted hexadecanoic acid and 1-(15-methyl-1-oxohexadecyl)-pyrrolidine as the most important metabolites. These compounds also exhibited high in silico binding affinity (−4.6 to −5.8 kcal/mol) to IL-1α, IL-1β, and IL-2. Results suggested the joint immuno-modulatory and anti-proliferative effect derived from selected compounds of underutilized marine food products such as ink. This is the first report of such biological activities in extracts from O. vulgaris ink.

Original languageEnglish
Article number112119
JournalFood and Chemical Toxicology
StatePublished - May 2021

Bibliographical note

Publisher Copyright:
© 2021 Elsevier Ltd


  • Anti-proliferative effect
  • Colorrectal cancer
  • Cytokine modulation
  • Ink
  • Metabolomic analysis
  • Octopus (Octopus vulgaris)


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