Biochemical characterization and inhibition of thermolabile hemolysin from Vibrio parahaemolyticus by phenolic compounds

Luis E. Vazquez-Morado, Ramon E. Robles-Zepeda, Adrian Ochoa-Leyva, Aldo A. Arvizu-Flores, Adriana Garibay-Escobar, Francisco Castillo-Yañez, Alonso A. Lopez-Zavala*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Vibrio parahaemolyticus (Vp), a typical microorganism inhabiting marine ecosystems, uses pathogenic virulence molecules such as hemolysins to cause bacterial infections of both human and marine animals. The thermolabile hemolysin VpTLH lyses human erythrocytes by a phospholipase B/A2 enzymatic activity in egg-yolk lecithin. However, few studies have been characterized the biochemical properties and the use of VpTLH as a molecular target for natural compounds as an alternative to control Vp infection. Here, we evaluated the biochemical and inhibition parameters of the recombinant VpTLH using enzymatic and hemolytic assays and determined the molecular interactions by in silico docking analysis. The highest enzymatic activity was at pH 8 and 50 C, and it was inactivated by 20 min at 60 C with Tm = 50.9 C. Additionally, the flavonoids quercetin, epigallocatechin gallate, and morin inhibited the VpTLH activity with IC50 values of 4.5 µM, 6.3 µM, and 9.9 µM, respectively; while phenolics acids were not effective inhibitors for this enzyme. Boltzmann and Arrhenius equation analysis indicate that VpTLH is a thermolabile enzyme. The inhibition of both enzymatic and hemolytic activities by flavonoids agrees with molecular docking, suggesting that flavonoids could interact with the active site’s amino acids. Future research is necessary to evaluate the antibacterial activity of flavonoids against Vp in vivo.

Original languageEnglish
Article number:e10506
Pages (from-to)e10506
JournalPeerJ
Volume9
DOIs
StatePublished - 6 Jan 2021

Bibliographical note

Funding Information:
This research was funded by the Universidad de Sonora grant: USO313004740, the UNAM-CIC-UNISON-2018 and -2019 grants for the academic exchange program. Luis E. Vazquez-Morado received a National Council on Science and Technology (CONACYT) M.Sc. scholarship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Funding Information:
We thank Cesar Otero-Le?n for technical support. This research was funded by the Universidad de Sonora grant: USO313004740, the UNAM-CIC-UNISON-2018 and -2019 grants for the academic exchange program. Luis E. Vazquez-Morado received a National Council on Science and Technology (CONACYT) M.Sc. scholarship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Publisher Copyright:
Copyright 2021 Vazquez-Morado et al.

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