Biological activities of organic fractions from Ficus obtusifolia and Haematoxylum brasiletto

Jorge Bello Martínez, Max Vidal Gutiérrez, Jose L. Rosas Acevedo, Luz P. Avila Caballero, Napoleón Navarro Tito, Felix Torres Guzmán, Ramón E. Robles Zepeda*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Ficus obtusifolia and Haematoxylum brasiletto are traditional Mexican medicine plants used to treat different types of diseases. Both species belong to genera recognized as a potential source of compounds with antioxidant and antiproliferative activity. This study focuses on identifying the organic fractions of hexane (Hx), dichloromethane (DCM), and ethyl acetate (EtOAc) of these two species for the potential isolation of antioxidant compounds and with antiproliferative activity. The antioxidant activity was evaluated by the DPPH and TEAC tests; for the quantification of phenolic compounds and flavonoids, the Folin-Ciocalteau and AlCl3 colorimetric tests were used, respectively. The antiproliferative activity was determined by the MTT assay on the A549, RAW264.7, and L929 cell lines. The DCM fraction of H. brasiletto was the sample that showed the highest antioxidant activity (299.7 mgEAG/g; DPPH IC50 of 60 µg/mL, and; TEAC IC50 of 1.9 µMTE/g) with antiproliferative activity (IC50 of 22.6 µg/mL and 23.8 µg/mL for A549 and RAW264.7, respectively). In F. obtusifolia only its EtOAc fraction showed antioxidant activity (DPPH IC50 of 55 µg/mL, and; TEAC IC50 of 1.8 µMTE/g), while its Hx fraction showed antiproliferative activity with an IC50 of 48.1 µg/mL and 42.2 µg/mL for A549 and RAW264.7, respectively. The results obtained show that the H. brasiletto EtOH extract, and its DCM fraction, showed potential as antioxidants and antiproliferative in vitro. Whereas of F. obtusifolia, only its Hx fraction showed antiproliferative potential in vitro.

Original languageEnglish
Pages (from-to)306-312
Number of pages7
JournalPharmacologyonline
Volume1
StatePublished - 30 Apr 2021

Bibliographical note

Publisher Copyright:
© 2021, SILAE (Italo-Latin American Society of Ethnomedicine). All rights reserved.

Keywords

  • Antioxidant activity
  • Antiproliferative activity
  • Ficus obtusifolia
  • Haematoxylum brasiletto

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