Breakthrough performance of large proteins on ion-exchange membrane columns

Rosa Maria Montesinos-Cisneros, Armando Lucero-Acuña, Jaime Ortega, Roberto Guzmán, Armando Tejeda-Mansir*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Protein adsorption of large proteins on ion-exchange membrane columns was theoretically and experimentally investigated using batch and fixed-bed systems. Thyroglobulin was used as the model protein. The study strongly suggests that part of the protein is physically retained inside the column during frontal mode operation. These experimental results were used to obtain a filtration function of the chromatographic system. In the theoretical analysis of the frontal protein adsorption, a model was integrated by the serial coupling of the membrane-transport model, the filtration model and the system-dispersion model. Two different techniques were employed in the estimation of the maximum adsorption capacity, the equilibrium desorption constant and the forward interaction rate constant, which are the parameters of the membrane-transport model. The fit of the model to the experimental data was not possible using the equilibrium parameters obtained in the batch experiments. The parameter estimation using a simplex optimization routine coupled to the solution of the partial differential model equations yields full prediction of the adsorption phenomena.

Original languageEnglish
Pages (from-to)117-125
Number of pages9
JournalBiotechnology and Applied Biochemistry
Issue number2
StatePublished - Oct 2007


  • Adsorption
  • Chromatography
  • Frontal analysis
  • Ion-exchange membrane columns
  • Protein breakthrough performance


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