Catalytic subunits atpα and atpβ from the Pacific white shrimp Litopenaeus vannamei FOF1 ATP-synthase complex: cDNA sequences, phylogenies, and mRNA quantification during hypoxia

Oliviert Martinez-Cruz; Fernando Garcia-Carreño; Arlett Robles-Romo; Alejandro Varela-Romero; Adriana Muhlia-Almazan

doi:10.1007/s10863-011-9340-0

Catalytic subunits atpα and atpβ from the Pacific white shrimp Litopenaeus vannamei F_OF₁ ATP-synthase complex: cDNA sequences, phylogenies, and mRNA quantification during hypoxia

Oliviert Martinez-Cruz, Fernando Garcia-Carreño, Arlett Robles-Romo, Alejandro Varela-Romero, Adriana Muhlia-Almazan^*

^*Corresponding author for this work

Departamento de Investigaciones Científicas y Tecnológicas

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

In the mitochondrial F_OF₁ ATP-synthase/ATPase complex, subunits α and β are part of the extrinsic portion that catalyses ATP synthesis. Since there are no reports about genes and proteins from these subunits in crustaceans, we analyzed the cDNA sequences of both subunits in the whiteleg shrimp Litopenaeus vannamei and their phylogenetic relationships. We also investigated the effect of hypoxia on shrimp by measuring changes in the mRNA amounts of atpα and atpβ. Our results confirmed highly conserved regions for both subunits and underlined unique features among others. The ATPβ deduced protein of shrimp was less conserved in size and sequence than ATPα. The relative mRNA amounts of atpα and atpβ changed in shrimp pleopods; hypoxia at 1.5 mg/L caused an increase in atpβ transcripts and a subsequent decrease when shrimp were re-oxygenated. Results confirm that changes in the mRNAs of the ATP-synthase subunits are part of the mechanisms allowing shrimp to deal with the metabolic adjustment displayed to tolerate hypoxia.

Original language	English
Pages (from-to)	119-133
Number of pages	15
Journal	Journal of Bioenergetics and Biomembranes
Volume	43
Issue number	2
DOIs	https://doi.org/10.1007/s10863-011-9340-0
State	Published - Apr 2011

Bibliographical note

Funding Information:
Acknowledgments AMA acknowledges support from Consejo Nacional de Ciencia y Tecnología (CONACYT National Council for Research and Technology, Mexico) for grant J48989-Z. OMC and ARR thank CONACYT for a graduate scholarship. We also thank Dr. Arturo Sanchez-Paz for critical reading of this manuscript.

Keywords

FFATP synthase
Hypoxia
Shrimp
atpα subunit
atpβ subunit

Access to Document

10.1007/s10863-011-9340-0

Cite this

Martinez-Cruz, O., Garcia-Carreño, F., Robles-Romo, A., Varela-Romero, A., & Muhlia-Almazan, A. (2011). Catalytic subunits atpα and atpβ from the Pacific white shrimp Litopenaeus vannamei F_OF₁ ATP-synthase complex: cDNA sequences, phylogenies, and mRNA quantification during hypoxia. Journal of Bioenergetics and Biomembranes, 43(2), 119-133. https://doi.org/10.1007/s10863-011-9340-0

@article{8c9e4c3bb3da4d6ea3cc698e3ead9687,

title = "Catalytic subunits atpα and atpβ from the Pacific white shrimp Litopenaeus vannamei FOF1 ATP-synthase complex: cDNA sequences, phylogenies, and mRNA quantification during hypoxia",

abstract = "In the mitochondrial FOF1 ATP-synthase/ATPase complex, subunits α and β are part of the extrinsic portion that catalyses ATP synthesis. Since there are no reports about genes and proteins from these subunits in crustaceans, we analyzed the cDNA sequences of both subunits in the whiteleg shrimp Litopenaeus vannamei and their phylogenetic relationships. We also investigated the effect of hypoxia on shrimp by measuring changes in the mRNA amounts of atpα and atpβ. Our results confirmed highly conserved regions for both subunits and underlined unique features among others. The ATPβ deduced protein of shrimp was less conserved in size and sequence than ATPα. The relative mRNA amounts of atpα and atpβ changed in shrimp pleopods; hypoxia at 1.5 mg/L caused an increase in atpβ transcripts and a subsequent decrease when shrimp were re-oxygenated. Results confirm that changes in the mRNAs of the ATP-synthase subunits are part of the mechanisms allowing shrimp to deal with the metabolic adjustment displayed to tolerate hypoxia.",

keywords = "FFATP synthase, Hypoxia, Shrimp, atpα subunit, atpβ subunit",

author = "Oliviert Martinez-Cruz and Fernando Garcia-Carre{\~n}o and Arlett Robles-Romo and Alejandro Varela-Romero and Adriana Muhlia-Almazan",

note = "Funding Information: Acknowledgments AMA acknowledges support from Consejo Nacional de Ciencia y Tecnolog{\'i}a (CONACYT National Council for Research and Technology, Mexico) for grant J48989-Z. OMC and ARR thank CONACYT for a graduate scholarship. We also thank Dr. Arturo Sanchez-Paz for critical reading of this manuscript.",

year = "2011",

month = apr,

doi = "10.1007/s10863-011-9340-0",

language = "Ingl{\'e}s",

volume = "43",

pages = "119--133",

journal = "Journal of Bioenergetics and Biomembranes",

issn = "0145-479X",

publisher = "Springer New York",

number = "2",

}

Martinez-Cruz, O, Garcia-Carreño, F, Robles-Romo, A, Varela-Romero, A & Muhlia-Almazan, A 2011, 'Catalytic subunits atpα and atpβ from the Pacific white shrimp Litopenaeus vannamei F_OF₁ ATP-synthase complex: cDNA sequences, phylogenies, and mRNA quantification during hypoxia', Journal of Bioenergetics and Biomembranes, vol. 43, no. 2, pp. 119-133. https://doi.org/10.1007/s10863-011-9340-0

Catalytic subunits atpα and atpβ from the Pacific white shrimp Litopenaeus vannamei F_OF₁ ATP-synthase complex: cDNA sequences, phylogenies, and mRNA quantification during hypoxia. / Martinez-Cruz, Oliviert; Garcia-Carreño, Fernando; Robles-Romo, Arlett et al.
In: Journal of Bioenergetics and Biomembranes, Vol. 43, No. 2, 04.2011, p. 119-133.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Catalytic subunits atpα and atpβ from the Pacific white shrimp Litopenaeus vannamei FOF1 ATP-synthase complex

T2 - cDNA sequences, phylogenies, and mRNA quantification during hypoxia

AU - Martinez-Cruz, Oliviert

AU - Garcia-Carreño, Fernando

AU - Robles-Romo, Arlett

AU - Varela-Romero, Alejandro

AU - Muhlia-Almazan, Adriana

N1 - Funding Information: Acknowledgments AMA acknowledges support from Consejo Nacional de Ciencia y Tecnología (CONACYT National Council for Research and Technology, Mexico) for grant J48989-Z. OMC and ARR thank CONACYT for a graduate scholarship. We also thank Dr. Arturo Sanchez-Paz for critical reading of this manuscript.

PY - 2011/4

Y1 - 2011/4

N2 - In the mitochondrial FOF1 ATP-synthase/ATPase complex, subunits α and β are part of the extrinsic portion that catalyses ATP synthesis. Since there are no reports about genes and proteins from these subunits in crustaceans, we analyzed the cDNA sequences of both subunits in the whiteleg shrimp Litopenaeus vannamei and their phylogenetic relationships. We also investigated the effect of hypoxia on shrimp by measuring changes in the mRNA amounts of atpα and atpβ. Our results confirmed highly conserved regions for both subunits and underlined unique features among others. The ATPβ deduced protein of shrimp was less conserved in size and sequence than ATPα. The relative mRNA amounts of atpα and atpβ changed in shrimp pleopods; hypoxia at 1.5 mg/L caused an increase in atpβ transcripts and a subsequent decrease when shrimp were re-oxygenated. Results confirm that changes in the mRNAs of the ATP-synthase subunits are part of the mechanisms allowing shrimp to deal with the metabolic adjustment displayed to tolerate hypoxia.

AB - In the mitochondrial FOF1 ATP-synthase/ATPase complex, subunits α and β are part of the extrinsic portion that catalyses ATP synthesis. Since there are no reports about genes and proteins from these subunits in crustaceans, we analyzed the cDNA sequences of both subunits in the whiteleg shrimp Litopenaeus vannamei and their phylogenetic relationships. We also investigated the effect of hypoxia on shrimp by measuring changes in the mRNA amounts of atpα and atpβ. Our results confirmed highly conserved regions for both subunits and underlined unique features among others. The ATPβ deduced protein of shrimp was less conserved in size and sequence than ATPα. The relative mRNA amounts of atpα and atpβ changed in shrimp pleopods; hypoxia at 1.5 mg/L caused an increase in atpβ transcripts and a subsequent decrease when shrimp were re-oxygenated. Results confirm that changes in the mRNAs of the ATP-synthase subunits are part of the mechanisms allowing shrimp to deal with the metabolic adjustment displayed to tolerate hypoxia.

KW - FFATP synthase

KW - Hypoxia

KW - Shrimp

KW - atpα subunit

KW - atpβ subunit

UR - http://www.scopus.com/inward/record.url?scp=79958243680&partnerID=8YFLogxK

U2 - 10.1007/s10863-011-9340-0

DO - 10.1007/s10863-011-9340-0

M3 - Artículo

SN - 0145-479X

VL - 43

SP - 119

EP - 133

JO - Journal of Bioenergetics and Biomembranes

JF - Journal of Bioenergetics and Biomembranes

IS - 2

ER -