cDNA cloning of the lysozyme of the white shrimp Penaeus vannamei

Rogerio R. Sotelo-Mundo, Maria A. Islas-Osuna, Enrique De-la-Re-Vega, Jorge Hernández-López, Francisco Vargas-Albores, Gloria Yepiz-Plascencia

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Abstract

Lysozyme, an antibacterial protein, has been implicated in innate immunity in invertebrates, but its activity in shrimp remained to be determined. We cloned the white shrimp lysozyme cDNA using a PCR strategy and detected its activity in haemocytes using a lytic-zone assay against Micrococcus luteus. The cloning was based on a reported EST (dbEST BE18831). The deduced amino acid sequence resulted in 150 amino with 46% identity to hen egg white lysozyme. RT-PCR was used to detect lysozyme mRNA in haemocytes. Analysis of the amino acid sequence of the shrimp lysozyme showed that it belongs to the C-type family of lysozymes. Furthermore, the lysozyme amino acid sequence contained extra residues at its C-terminus, which are characteristic of marine invertebrates. This information will be useful in future studies on the molecular mechanisms of immunity in marine invertebrates. © 2003 Elsevier Ltd. All rights reserved.
Original languageAmerican English
Pages (from-to)325-331
Number of pages7
JournalFish and Shellfish Immunology
DOIs
StatePublished - 1 Jan 2003

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    Sotelo-Mundo, R. R., Islas-Osuna, M. A., De-la-Re-Vega, E., Hernández-López, J., Vargas-Albores, F., & Yepiz-Plascencia, G. (2003). cDNA cloning of the lysozyme of the white shrimp Penaeus vannamei. Fish and Shellfish Immunology, 325-331. https://doi.org/10.1016/S1050-4648(02)00176-6