Characterization and expression of prohibitin during the mexican bean weevil (Zabrotes subfasciatus, Boheman, 1833) larvae development

Diana Villegas-Coronado, Ana María Guzman-Partida*, Emmanuel Aispuro-Hernandez, Luz Vazquez-Moreno, José Ángel Huerta-Ocampo, José Andre i. Sarabia-Sainz, Nayelli Guadalupe Teran-Saavedra, Christian Minjarez-Osorio, Reina Castro-Longoria, Amir Maldonado, Irlanda Lagarda-Diaz

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Prohibitin (PHB) is a highly conserved eukaryotic protein complex involved in multiple cellular processes. In insects, PHB has been identified as a potential target protein to insecticidal molecules acting as a receptor of PF2 insecticidal lectin in the midgut of Zabrotes subfasciatus larvae (bean pest) and Cry protein of Bacillus thuringiensis in Leptinotarsa decemlineata (Colorado potato beetle). This study aimed to characterize the structural features of Z. subfasciatus prohibitin (ZsPHB) by homology modeling and evaluate its expression and tissue localization at different stages of larval development both at the transcript and protein levels. The samples were collected from eggs and larvae of different developmental stages. The immunodetection of ZsPHB was done with anti-PHB1 and confirmed by LC-MS/MS analysis. Gene expression analysis of ZsPHB1 and ZsPHB2 was performed by RT-qPCR, and immunohistochemistry with FITC-labeled anti-PHB1. Results showed that ZsPHBs exhibit distinctive characteristics of the SPFH protein superfamily. The transcript levels suggest a coordinated expression of ZsPHB1 and ZsPHB2 genes, while ZsPHB1 was detected in soluble protein extracts depending on the stage of development. Histological examination showed ZsPHB1 is present in all larval tissues, with an intense fluorescence signal observed at the gut. These results suggest a physiologically important role of PHB during Z. subfasciatus development and show its regulation occurs at the transcriptional and post-transcriptional levels. This is the first characterization of PHB in Z. subfasciatus.

Original languageEnglish
Article number110770
JournalComparative Biochemistry and Physiology Part - B: Biochemistry and Molecular Biology
Volume262
DOIs
StatePublished - 1 Oct 2022
Externally publishedYes

Bibliographical note

Funding Information:
The authors thank to Cátedras-CONACyT program.

Publisher Copyright:
© 2022 Elsevier Inc.

Keywords

  • Histological detection
  • Insect larvae
  • Prohibitin
  • Structural features
  • Transcript levels
  • Zabrotes subfasciatus

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