Characterization of Diarreaghenic Escherichia coli Strains Isolated from Healthy Donors, including a Triple Hybrid Strain

Evelyn Méndez-Moreno, Liliana Caporal-Hernandez, Pablo A. Mendez-Pfeiffer, Yessica Enciso-Martinez, Rafael De la Rosa López, Dora Valencia, Margarita M.P. Arenas-Hernández, Manuel G. Ballesteros-Monrreal*, Edwin Barrios-Villa

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Escherichia coli is a well-recognized inhabitant of the animal and human gut. Its presence represents an essential component of the microbiome. There are six pathogenic variants of E. coli associated with diarrheal processes, known as pathotypes. These harbor genetic determinants that allow them to be classified as such. In this work, we report the presence of diarrheagenic pathotypes of E. coli strains isolated from healthy donors. Ninety E. coli strains were analyzed, of which forty-six (51%) harbored virulence markers specifics for diarrheagenic pathotypes, including four hybrids (one of them with genetic determinants of three DEC pathotypes). We also identified phylogenetic groups with a higher prevalence of B2 (45.6%) and A (17.8%). In addition, resistance to sulfonamides (100%), and aminoglycosides (100%) was found in 100% of the strains, with a lower prevalence of resistance to cefotaxime (13.3%), ceftriaxone (12.2%), fosfomycin (10%), and meropenem (0%). All analyzed strains were classified as multidrug resistant. Virulence genes were also investigated, which led us to propose three new virotypes. Among the virulence traits observed, the ability to form biofilms stands out, which was superior to that of the E. coli and Staphylococcus aureus strains used as positive controls.

Original languageEnglish
Article number833
JournalAntibiotics
Volume11
Issue number7
DOIs
StatePublished - Jul 2022

Bibliographical note

Funding Information:
Funding: This work was supported by División de Ciencias e Ingeniería, Unidad Regional Norte, Universidad de Sonora internal project number USO413007345, and Programa de Apoyo a la Incor-poración de Nuevos Profesores de Tiempo Completo (PRODEP), Secretaría de Educación Pública, México, number 257529.

Funding Information:
This work was supported by División de Ciencias e Ingeniería, Unidad Regional Norte, Universidad de Sonora internal project number USO413007345, and Programa de Apoyo a la Incorporación de Nuevos Profesores de Tiempo Completo (PRODEP), Secretaría de Educación Pública, México, number 257529.

Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

  • Escherichia coli
  • antimicrobial resistance
  • healthy donors
  • pathotypes

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