Characterization of the jumbo squid (dosidicus gigas) skin by-product by shotgun proteomics and protein-based bioinformatics

Mónica Carrera*, Josafat Marina Ezquerra-Brauer, Santiago P. Aubourg

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Jumbo squid (Dosidicus gigas) is one of the largest cephalopods, and represents an important economic fishery in several regions of the Pacific Ocean, from southern California in the United States to southern Chile. Large and considerable discards of this species, such as skin, have been reported to constitute an important source of potential by-products. In this paper, a shotgun proteomics approach was applied for the first time to the characterization of the jumbo squid (Dosidicus gigas) skin proteome. A total of 1004 different peptides belonging to 219 different proteins were identified. The final proteome compilation was investigated by integrated in-silico studies, including gene ontology (GO) term enrichment, pathways, and networks studies. Potential new valuable bioactive peptides such as antimicrobial, bioactive collagen peptides, antihypertensive and antitumoral peptides were predicted to be present in the jumbo squid skin proteome. The integration of the global proteomics results and the bioinformatics analysis of the jumbo squid skin proteome show a comprehensive knowledge of this fishery discard and provide potential bioactive peptides of this marine by-product.

Original languageEnglish
Article number31
JournalMarine Drugs
Volume18
Issue number1
DOIs
StatePublished - 1 Jan 2020
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2019 by the authors.

Keywords

  • Bioactive peptides
  • By-product
  • Dosidicus gigas
  • Mass spectrometry
  • Protein-based bioinformatics
  • Shotgun proteomics
  • Skin
  • Squid

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