Chemical composition, fatty acid profile and molecular changes derived from nitrogen stress in the diatom Chaetoceros muelleri

Damaristelma de Jesús-Campos; José Antonio López-Elías; Luis Ángel Medina-Juarez; Gisela Carvallo-Ruiz; Diana Fimbres-Olivarria; Corina Hayano-Kanashiro

doi:10.1016/j.aqrep.2020.100281

Chemical composition, fatty acid profile and molecular changes derived from nitrogen stress in the diatom Chaetoceros muelleri

Damaristelma de Jesús-Campos, José Antonio López-Elías, Luis Ángel Medina-Juarez, Gisela Carvallo-Ruiz, Diana Fimbres-Olivarria^*, Corina Hayano-Kanashiro

^*Corresponding author for this work

Departamento de Investigaciones Científicas y Tecnológicas

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2 Scopus citations

Abstract

Microalgae have great potential as biofactories for producing a wide variety of valuable products. Under stress conditions, it is well-known that microalgae accumulate various organic compounds. However, there is little information on the response of the diatom Chaetoceros muelleri to different nitrogen concentrations. In the present study, C. muelleri was grown in medium containing different concentrations of nitrogen (NaNO₃; f: 1.76 mM, f/4: 0.44 mM, f/10: 0.18 mM, and 2f: 3.53 mM) and their proximal composition, fatty acid profile, and gene expression (AMT1, PTD9, GltD, DGAT2, and NRT2) compared. The results showed no significant differences in biomass content among nitrogen treatments. Treatment f/10 significantly increased accumulation of lipids and carbohydrates but lowered the concentration of proteins. This treatment also produced shorter-chain fatty acids and a low percentage of polyunsaturated fatty acids, demonstrating the potential of this medium for growing microalgae with a suitable fatty acid profile for biodiesel production. Furthermore, DGAT2 and NRT2 expression was highest at lower nitrogen concentrations, suggesting these genes could be important for modulating lipid biosynthesis in response to nitrogen limitation.

Original language	English
Article number	100281
Journal	Aquaculture Reports
Volume	16
DOIs	https://doi.org/10.1016/j.aqrep.2020.100281
State	Published - Mar 2020

Bibliographical note

Funding Information:
The present research was funded by the National Council of Science and Technology (Mexico)-Consejo Nacional de Ciencia y Tecnología (CONACyT project Nº 000000000257155). The authors thank Dr. Juan Carlos Ramírez-Suárez (Laboratorio de Productos Pesqueros. Centro de Investigación en Alimentación y Desarrollo, A.C [CIAD]) for allowing the fatty acid analysis in their facilities. D. de Jesus-Campos was also funded by a Master Fellowship from CONACyT (Nº 638640 ).

Funding Information:
The present research was funded by the National Council of Science and Technology (Mexico)-Consejo Nacional de Ciencia y Tecnolog?a (CONACyT project N? 000000000257155). The authors thank Dr. Juan Carlos Ram?rez-Su?rez (Laboratorio de Productos Pesqueros. Centro de Investigaci?n en Alimentaci?n y Desarrollo, A.C [CIAD]) for allowing the fatty acid analysis in their facilities. D. de Jesus-Campos was also funded by a Master Fellowship from CONACyT (N? 638640).

Publisher Copyright:
© 2020 The Authors

Keywords

Biochemical composition
Chaetoceros muelleri
Fatty acid methyl esters
Gene expression
Nitrogen stress

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10.1016/j.aqrep.2020.100281

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title = "Chemical composition, fatty acid profile and molecular changes derived from nitrogen stress in the diatom Chaetoceros muelleri",

abstract = "Microalgae have great potential as biofactories for producing a wide variety of valuable products. Under stress conditions, it is well-known that microalgae accumulate various organic compounds. However, there is little information on the response of the diatom Chaetoceros muelleri to different nitrogen concentrations. In the present study, C. muelleri was grown in medium containing different concentrations of nitrogen (NaNO3; f: 1.76 mM, f/4: 0.44 mM, f/10: 0.18 mM, and 2f: 3.53 mM) and their proximal composition, fatty acid profile, and gene expression (AMT1, PTD9, GltD, DGAT2, and NRT2) compared. The results showed no significant differences in biomass content among nitrogen treatments. Treatment f/10 significantly increased accumulation of lipids and carbohydrates but lowered the concentration of proteins. This treatment also produced shorter-chain fatty acids and a low percentage of polyunsaturated fatty acids, demonstrating the potential of this medium for growing microalgae with a suitable fatty acid profile for biodiesel production. Furthermore, DGAT2 and NRT2 expression was highest at lower nitrogen concentrations, suggesting these genes could be important for modulating lipid biosynthesis in response to nitrogen limitation.",

keywords = "Biochemical composition, Chaetoceros muelleri, Fatty acid methyl esters, Gene expression, Nitrogen stress",

author = "{de Jes{\'u}s-Campos}, Damaristelma and L{\'o}pez-El{\'i}as, {Jos{\'e} Antonio} and Medina-Juarez, {Luis {\'A}ngel} and Gisela Carvallo-Ruiz and Diana Fimbres-Olivarria and Corina Hayano-Kanashiro",

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year = "2020",

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doi = "10.1016/j.aqrep.2020.100281",

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volume = "16",

journal = "Aquaculture Reports",

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Chemical composition, fatty acid profile and molecular changes derived from nitrogen stress in the diatom Chaetoceros muelleri. / de Jesús-Campos, Damaristelma; López-Elías, José Antonio; Medina-Juarez, Luis Ángel; Carvallo-Ruiz, Gisela; Fimbres-Olivarria, Diana ; Hayano-Kanashiro, Corina.

In: Aquaculture Reports, Vol. 16, 100281, 03.2020.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Chemical composition, fatty acid profile and molecular changes derived from nitrogen stress in the diatom Chaetoceros muelleri

AU - de Jesús-Campos, Damaristelma

AU - López-Elías, José Antonio

AU - Medina-Juarez, Luis Ángel

AU - Carvallo-Ruiz, Gisela

AU - Fimbres-Olivarria, Diana

AU - Hayano-Kanashiro, Corina

PY - 2020/3

Y1 - 2020/3

N2 - Microalgae have great potential as biofactories for producing a wide variety of valuable products. Under stress conditions, it is well-known that microalgae accumulate various organic compounds. However, there is little information on the response of the diatom Chaetoceros muelleri to different nitrogen concentrations. In the present study, C. muelleri was grown in medium containing different concentrations of nitrogen (NaNO3; f: 1.76 mM, f/4: 0.44 mM, f/10: 0.18 mM, and 2f: 3.53 mM) and their proximal composition, fatty acid profile, and gene expression (AMT1, PTD9, GltD, DGAT2, and NRT2) compared. The results showed no significant differences in biomass content among nitrogen treatments. Treatment f/10 significantly increased accumulation of lipids and carbohydrates but lowered the concentration of proteins. This treatment also produced shorter-chain fatty acids and a low percentage of polyunsaturated fatty acids, demonstrating the potential of this medium for growing microalgae with a suitable fatty acid profile for biodiesel production. Furthermore, DGAT2 and NRT2 expression was highest at lower nitrogen concentrations, suggesting these genes could be important for modulating lipid biosynthesis in response to nitrogen limitation.

AB - Microalgae have great potential as biofactories for producing a wide variety of valuable products. Under stress conditions, it is well-known that microalgae accumulate various organic compounds. However, there is little information on the response of the diatom Chaetoceros muelleri to different nitrogen concentrations. In the present study, C. muelleri was grown in medium containing different concentrations of nitrogen (NaNO3; f: 1.76 mM, f/4: 0.44 mM, f/10: 0.18 mM, and 2f: 3.53 mM) and their proximal composition, fatty acid profile, and gene expression (AMT1, PTD9, GltD, DGAT2, and NRT2) compared. The results showed no significant differences in biomass content among nitrogen treatments. Treatment f/10 significantly increased accumulation of lipids and carbohydrates but lowered the concentration of proteins. This treatment also produced shorter-chain fatty acids and a low percentage of polyunsaturated fatty acids, demonstrating the potential of this medium for growing microalgae with a suitable fatty acid profile for biodiesel production. Furthermore, DGAT2 and NRT2 expression was highest at lower nitrogen concentrations, suggesting these genes could be important for modulating lipid biosynthesis in response to nitrogen limitation.

KW - Biochemical composition

KW - Chaetoceros muelleri

KW - Fatty acid methyl esters

KW - Gene expression

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JF - Aquaculture Reports

SN - 2352-5134

M1 - 100281

ER -

Chemical composition, fatty acid profile and molecular changes derived from nitrogen stress in the diatom Chaetoceros muelleri

Abstract

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Keywords

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