Chemochromic properties of neutral polyaniline throughout cholesterol exposure

C. L.Medrano Pesqueira, T. Del Castillo-Castro*, M. M. Castillo-Ortega, J. C. Encinas

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


This paper presents a novel biosensing platform for cholesterol accomplished by the spin-coating deposition of neutral polyaniline on poly(methyl methacrylate) support and the subsequent covalent immobilization of cholesterol oxidase onto polyaniline surface. The polyaniline film entraps the enzyme and it also acts as an active layer for optical sensing of free cholesterol. The effects of immobilization process of the enzyme over the oxidation state of polyaniline backbone and the optimum pH and temperature conditions for immobilized cholesterol oxidase were studied. The sensibility of the system to cholesterol in concentrations equivalent to those present in human serum evidences its potentiality in an optical method simply based on chemochromic properties of polyaniline.

Original languageEnglish
Article number71
JournalJournal of Polymer Research
Issue number2
StatePublished - Feb 2013


  • Biosensing platform
  • Chemochromic properties
  • Cholesterol
  • Polyaniline


Dive into the research topics of 'Chemochromic properties of neutral polyaniline throughout cholesterol exposure'. Together they form a unique fingerprint.

Cite this