Chemochromic properties of neutral polyaniline throughout cholesterol exposure

C. L.Medrano Pesqueira; T. Del Castillo-Castro; M. M. Castillo-Ortega; J. C. Encinas

doi:10.1007/s10965-012-0071-7

Chemochromic properties of neutral polyaniline throughout cholesterol exposure

C. L.Medrano Pesqueira, T. Del Castillo-Castro^*, M. M. Castillo-Ortega, J. C. Encinas

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

This paper presents a novel biosensing platform for cholesterol accomplished by the spin-coating deposition of neutral polyaniline on poly(methyl methacrylate) support and the subsequent covalent immobilization of cholesterol oxidase onto polyaniline surface. The polyaniline film entraps the enzyme and it also acts as an active layer for optical sensing of free cholesterol. The effects of immobilization process of the enzyme over the oxidation state of polyaniline backbone and the optimum pH and temperature conditions for immobilized cholesterol oxidase were studied. The sensibility of the system to cholesterol in concentrations equivalent to those present in human serum evidences its potentiality in an optical method simply based on chemochromic properties of polyaniline.

Original language	English
Article number	71
Journal	Journal of Polymer Research
Volume	20
Issue number	2
DOIs	https://doi.org/10.1007/s10965-012-0071-7
State	Published - Feb 2013

Keywords

Biosensing platform
Chemochromic properties
Cholesterol
Polyaniline

Access to Document

10.1007/s10965-012-0071-7

Cite this

@article{9a63b69b39ef420ea620c4f4cffb7e1d,

title = "Chemochromic properties of neutral polyaniline throughout cholesterol exposure",

abstract = "This paper presents a novel biosensing platform for cholesterol accomplished by the spin-coating deposition of neutral polyaniline on poly(methyl methacrylate) support and the subsequent covalent immobilization of cholesterol oxidase onto polyaniline surface. The polyaniline film entraps the enzyme and it also acts as an active layer for optical sensing of free cholesterol. The effects of immobilization process of the enzyme over the oxidation state of polyaniline backbone and the optimum pH and temperature conditions for immobilized cholesterol oxidase were studied. The sensibility of the system to cholesterol in concentrations equivalent to those present in human serum evidences its potentiality in an optical method simply based on chemochromic properties of polyaniline.",

keywords = "Biosensing platform, Chemochromic properties, Cholesterol, Polyaniline",

author = "Pesqueira, {C. L.Medrano} and {Del Castillo-Castro}, T. and Castillo-Ortega, {M. M.} and Encinas, {J. C.}",

year = "2013",

month = feb,

doi = "10.1007/s10965-012-0071-7",

language = "Ingl{\'e}s",

volume = "20",

journal = "Journal of Polymer Research",

issn = "1022-9760",

publisher = "Springer Netherlands",

number = "2",

}

TY - JOUR

T1 - Chemochromic properties of neutral polyaniline throughout cholesterol exposure

AU - Pesqueira, C. L.Medrano

AU - Del Castillo-Castro, T.

AU - Castillo-Ortega, M. M.

AU - Encinas, J. C.

PY - 2013/2

Y1 - 2013/2

N2 - This paper presents a novel biosensing platform for cholesterol accomplished by the spin-coating deposition of neutral polyaniline on poly(methyl methacrylate) support and the subsequent covalent immobilization of cholesterol oxidase onto polyaniline surface. The polyaniline film entraps the enzyme and it also acts as an active layer for optical sensing of free cholesterol. The effects of immobilization process of the enzyme over the oxidation state of polyaniline backbone and the optimum pH and temperature conditions for immobilized cholesterol oxidase were studied. The sensibility of the system to cholesterol in concentrations equivalent to those present in human serum evidences its potentiality in an optical method simply based on chemochromic properties of polyaniline.

AB - This paper presents a novel biosensing platform for cholesterol accomplished by the spin-coating deposition of neutral polyaniline on poly(methyl methacrylate) support and the subsequent covalent immobilization of cholesterol oxidase onto polyaniline surface. The polyaniline film entraps the enzyme and it also acts as an active layer for optical sensing of free cholesterol. The effects of immobilization process of the enzyme over the oxidation state of polyaniline backbone and the optimum pH and temperature conditions for immobilized cholesterol oxidase were studied. The sensibility of the system to cholesterol in concentrations equivalent to those present in human serum evidences its potentiality in an optical method simply based on chemochromic properties of polyaniline.

KW - Biosensing platform

KW - Chemochromic properties

KW - Cholesterol

KW - Polyaniline

UR - http://www.scopus.com/inward/record.url?scp=84871858887&partnerID=8YFLogxK

U2 - 10.1007/s10965-012-0071-7

DO - 10.1007/s10965-012-0071-7

M3 - Artículo

SN - 1022-9760

VL - 20

JO - Journal of Polymer Research

JF - Journal of Polymer Research

IS - 2

M1 - 71

ER -

Chemochromic properties of neutral polyaniline throughout cholesterol exposure

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this