TY - JOUR
T1 - Chitosan-Coated Silver Nanoparticles Inhibit Adherence and Biofilm Formation of Uropathogenic Escherichia coli
AU - Mendez-Pfeiffer, Pablo
AU - Ballesteros-Monrreal, Manuel G.
AU - Juarez, Josue
AU - Gastelum-Cabrera, Marisol
AU - Martinez-Flores, Patricia
AU - Taboada, Pablo
AU - Valencia, Dora
N1 - Publisher Copyright:
© 2024 The Authors. Published by American Chemical Society.
PY - 2024/4/12
Y1 - 2024/4/12
N2 - Urinary tract infections are commonly caused by uropathogenic Escherichia coli (UPEC), which usually presents multiple virulence and resistance mechanisms, making it difficult to treat. It has been demonstrated that silver and polymeric nanoparticles had potential against these pathogens. In this study, we synthesized thiol chitosan-coated silver nanoparticles (SH-Cs-AgNPs) and evaluated their antibacterial, antibiofilm and antiadherence activity against clinical isolates of UPEC. The SH-Cs-AgNPs showed a spherical shape with a size of 17.80 ± 2.67 nm and zeta potential of 18 ± 2 mV. We observed a potent antibacterial and antibiofilm activity as low as 12.5 μg/mL, as well as a reduction in the adherence of UPEC to mammalian cells at concentrations of 1.06 and 0.53 μg/mL. These findings demonstrate that SH-Cs-AgNPs have potential as a new therapeutic compound against infections caused by UPEC.
AB - Urinary tract infections are commonly caused by uropathogenic Escherichia coli (UPEC), which usually presents multiple virulence and resistance mechanisms, making it difficult to treat. It has been demonstrated that silver and polymeric nanoparticles had potential against these pathogens. In this study, we synthesized thiol chitosan-coated silver nanoparticles (SH-Cs-AgNPs) and evaluated their antibacterial, antibiofilm and antiadherence activity against clinical isolates of UPEC. The SH-Cs-AgNPs showed a spherical shape with a size of 17.80 ± 2.67 nm and zeta potential of 18 ± 2 mV. We observed a potent antibacterial and antibiofilm activity as low as 12.5 μg/mL, as well as a reduction in the adherence of UPEC to mammalian cells at concentrations of 1.06 and 0.53 μg/mL. These findings demonstrate that SH-Cs-AgNPs have potential as a new therapeutic compound against infections caused by UPEC.
KW - antiadherence activity
KW - antibacterial activity
KW - chitosan-silver nanoparticles
KW - uropathogenic Escherichia coli
UR - http://www.scopus.com/inward/record.url?scp=85184919344&partnerID=8YFLogxK
U2 - 10.1021/acsinfecdis.3c00229
DO - 10.1021/acsinfecdis.3c00229
M3 - Artículo
C2 - 38287229
AN - SCOPUS:85184919344
SN - 2373-8227
VL - 10
SP - 1126
EP - 1136
JO - ACS Infectious Diseases
JF - ACS Infectious Diseases
IS - 4
ER -