Chitosan/carrageenan/lysozyme particles: Synthesis, characterization and antifungal activity against aspergillus parasiticus

C. N. Hernández-Téllez; M. O. Cortez-Rocha; A. Burgos Hernández; E. C. Rosas-Burgos; J. Lizardi-Mendoza; W. Torres-Arreola; M. G. Burboa-Zazueta; M. Plascencia-Jatomea

doi:10.24275/uam/izt/dcbi/revmexingquim/2018v17n3/Hernandez

Chitosan/carrageenan/lysozyme particles: Synthesis, characterization and antifungal activity against aspergillus parasiticus

C. N. Hernández-Téllez, M. O. Cortez-Rocha, A. Burgos Hernández, E. C. Rosas-Burgos, J. Lizardi-Mendoza, W. Torres-Arreola, M. G. Burboa-Zazueta, M. Plascencia-Jatomea^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

In this study, particles of chitosan-carrageenan (Cs-Crg) and chitosan-carrageenan-lysozyme (CS-Crg-LZ) were synthesized by ionic complexation. The particles were analyzed by atomic force microscopy, dynamic light scattering and FT-IR, and the percentage of association of lysozyme was determined. The biological activity of the particles was evaluated by viability assays of Aspergillus parasiticus spores and cytotoxicity in ARPE-19 cells. The effect on subcellular structures was analyzed by fluorescence microscopy. The addition of lysozyme reduced the size of the particles. The size and Z potential of the synthesized particles were 725±5.5 and 250±8.1 nm and 28.7±1.0 and 21.6±0.8 mV for CS-Crg and CS-Crg-LZ, respectively. CS-Crg-LZ and CS-Crg particles exhibited a higher (p<0.05) inhibitory effect on the viability of A. parasiticus with respect to control, which increased proportionally to the particles concentration. None of the particles showed a mutagenic effect and they did not present cytotoxicity in ARPE-19 cells; likewise, no changes were observed in cell morphology with respect to control, observing normal structures in the actin cytoskeleton and in the DNA structure. These results show that chitosan-based particles are a feasible and safe option for the control of pathogenic fungi of agricultural-food interest.

Translated title of the contribution	Partículas de quitosano/carragenina/lisozima: Síntesis, caracterización y actividad antifúngica contra aspergillus parasiticus
Original language	English
Pages (from-to)	897-912
Number of pages	16
Journal	Revista Mexicana de Ingeniera Quimica
Volume	17
Issue number	3
DOIs	https://doi.org/10.24275/uam/izt/dcbi/revmexingquim/2018v17n3/Hernandez
State	Published - 1 Sep 2018

Bibliographical note

Publisher Copyright:
© 2018, Universidad Autonoma Metropolitana Iztapalapa. All rights reserved.

Keywords

Biocomposites
Carrageenan
Cytotoxicity
Protein release
Subcellular structures

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10.24275/uam/izt/dcbi/revmexingquim/2018v17n3/Hernandez

Cite this

Hernández-Téllez, C. N., Cortez-Rocha, M. O., Burgos Hernández, A., Rosas-Burgos, E. C., Lizardi-Mendoza, J., Torres-Arreola, W., Burboa-Zazueta, M. G., & Plascencia-Jatomea, M. (2018). Chitosan/carrageenan/lysozyme particles: Synthesis, characterization and antifungal activity against aspergillus parasiticus. Revista Mexicana de Ingeniera Quimica, 17(3), 897-912. https://doi.org/10.24275/uam/izt/dcbi/revmexingquim/2018v17n3/Hernandez

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title = "Chitosan/carrageenan/lysozyme particles: Synthesis, characterization and antifungal activity against aspergillus parasiticus",

abstract = "In this study, particles of chitosan-carrageenan (Cs-Crg) and chitosan-carrageenan-lysozyme (CS-Crg-LZ) were synthesized by ionic complexation. The particles were analyzed by atomic force microscopy, dynamic light scattering and FT-IR, and the percentage of association of lysozyme was determined. The biological activity of the particles was evaluated by viability assays of Aspergillus parasiticus spores and cytotoxicity in ARPE-19 cells. The effect on subcellular structures was analyzed by fluorescence microscopy. The addition of lysozyme reduced the size of the particles. The size and Z potential of the synthesized particles were 725±5.5 and 250±8.1 nm and 28.7±1.0 and 21.6±0.8 mV for CS-Crg and CS-Crg-LZ, respectively. CS-Crg-LZ and CS-Crg particles exhibited a higher (p<0.05) inhibitory effect on the viability of A. parasiticus with respect to control, which increased proportionally to the particles concentration. None of the particles showed a mutagenic effect and they did not present cytotoxicity in ARPE-19 cells; likewise, no changes were observed in cell morphology with respect to control, observing normal structures in the actin cytoskeleton and in the DNA structure. These results show that chitosan-based particles are a feasible and safe option for the control of pathogenic fungi of agricultural-food interest.",

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Hernández-Téllez, CN, Cortez-Rocha, MO , Burgos Hernández, A , Rosas-Burgos, EC, Lizardi-Mendoza, J, Torres-Arreola, W, Burboa-Zazueta, MG & Plascencia-Jatomea, M 2018, 'Chitosan/carrageenan/lysozyme particles: Synthesis, characterization and antifungal activity against aspergillus parasiticus', Revista Mexicana de Ingeniera Quimica, vol. 17, no. 3, pp. 897-912. https://doi.org/10.24275/uam/izt/dcbi/revmexingquim/2018v17n3/Hernandez

TY - JOUR

T1 - Chitosan/carrageenan/lysozyme particles

T2 - Synthesis, characterization and antifungal activity against aspergillus parasiticus

AU - Hernández-Téllez, C. N.

AU - Cortez-Rocha, M. O.

AU - Burgos Hernández, A.

AU - Rosas-Burgos, E. C.

AU - Lizardi-Mendoza, J.

AU - Torres-Arreola, W.

AU - Burboa-Zazueta, M. G.

AU - Plascencia-Jatomea, M.

PY - 2018/9/1

Y1 - 2018/9/1

N2 - In this study, particles of chitosan-carrageenan (Cs-Crg) and chitosan-carrageenan-lysozyme (CS-Crg-LZ) were synthesized by ionic complexation. The particles were analyzed by atomic force microscopy, dynamic light scattering and FT-IR, and the percentage of association of lysozyme was determined. The biological activity of the particles was evaluated by viability assays of Aspergillus parasiticus spores and cytotoxicity in ARPE-19 cells. The effect on subcellular structures was analyzed by fluorescence microscopy. The addition of lysozyme reduced the size of the particles. The size and Z potential of the synthesized particles were 725±5.5 and 250±8.1 nm and 28.7±1.0 and 21.6±0.8 mV for CS-Crg and CS-Crg-LZ, respectively. CS-Crg-LZ and CS-Crg particles exhibited a higher (p<0.05) inhibitory effect on the viability of A. parasiticus with respect to control, which increased proportionally to the particles concentration. None of the particles showed a mutagenic effect and they did not present cytotoxicity in ARPE-19 cells; likewise, no changes were observed in cell morphology with respect to control, observing normal structures in the actin cytoskeleton and in the DNA structure. These results show that chitosan-based particles are a feasible and safe option for the control of pathogenic fungi of agricultural-food interest.

AB - In this study, particles of chitosan-carrageenan (Cs-Crg) and chitosan-carrageenan-lysozyme (CS-Crg-LZ) were synthesized by ionic complexation. The particles were analyzed by atomic force microscopy, dynamic light scattering and FT-IR, and the percentage of association of lysozyme was determined. The biological activity of the particles was evaluated by viability assays of Aspergillus parasiticus spores and cytotoxicity in ARPE-19 cells. The effect on subcellular structures was analyzed by fluorescence microscopy. The addition of lysozyme reduced the size of the particles. The size and Z potential of the synthesized particles were 725±5.5 and 250±8.1 nm and 28.7±1.0 and 21.6±0.8 mV for CS-Crg and CS-Crg-LZ, respectively. CS-Crg-LZ and CS-Crg particles exhibited a higher (p<0.05) inhibitory effect on the viability of A. parasiticus with respect to control, which increased proportionally to the particles concentration. None of the particles showed a mutagenic effect and they did not present cytotoxicity in ARPE-19 cells; likewise, no changes were observed in cell morphology with respect to control, observing normal structures in the actin cytoskeleton and in the DNA structure. These results show that chitosan-based particles are a feasible and safe option for the control of pathogenic fungi of agricultural-food interest.

KW - Biocomposites

KW - Carrageenan

KW - Cytotoxicity

KW - Protein release

KW - Subcellular structures

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U2 - 10.24275/uam/izt/dcbi/revmexingquim/2018v17n3/Hernandez

DO - 10.24275/uam/izt/dcbi/revmexingquim/2018v17n3/Hernandez

M3 - Artículo

SN - 1665-2738

VL - 17

SP - 897

EP - 912

JO - Revista Mexicana de Ingeniera Quimica

JF - Revista Mexicana de Ingeniera Quimica

IS - 3

ER -

Hernández-Téllez CN, Cortez-Rocha MO , Burgos Hernández A , Rosas-Burgos EC, Lizardi-Mendoza J, Torres-Arreola W et al. Chitosan/carrageenan/lysozyme particles: Synthesis, characterization and antifungal activity against aspergillus parasiticus. Revista Mexicana de Ingeniera Quimica. 2018 Sep 1;17(3):897-912. doi: 10.24275/uam/izt/dcbi/revmexingquim/2018v17n3/Hernandez

Chitosan/carrageenan/lysozyme particles: Synthesis, characterization and antifungal activity against aspergillus parasiticus

Abstract

Bibliographical note

Keywords

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