Chitosan/carrageenan/lysozyme particles: Synthesis, characterization and antifungal activity against aspergillus parasiticus

C. N. Hernández-Téllez, M. O. Cortez-Rocha, A. Burgos Hernández, E. C. Rosas-Burgos, J. Lizardi-Mendoza, W. Torres-Arreola, M. G. Burboa-Zazueta, M. Plascencia-Jatomea*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

In this study, particles of chitosan-carrageenan (Cs-Crg) and chitosan-carrageenan-lysozyme (CS-Crg-LZ) were synthesized by ionic complexation. The particles were analyzed by atomic force microscopy, dynamic light scattering and FT-IR, and the percentage of association of lysozyme was determined. The biological activity of the particles was evaluated by viability assays of Aspergillus parasiticus spores and cytotoxicity in ARPE-19 cells. The effect on subcellular structures was analyzed by fluorescence microscopy. The addition of lysozyme reduced the size of the particles. The size and Z potential of the synthesized particles were 725±5.5 and 250±8.1 nm and 28.7±1.0 and 21.6±0.8 mV for CS-Crg and CS-Crg-LZ, respectively. CS-Crg-LZ and CS-Crg particles exhibited a higher (p<0.05) inhibitory effect on the viability of A. parasiticus with respect to control, which increased proportionally to the particles concentration. None of the particles showed a mutagenic effect and they did not present cytotoxicity in ARPE-19 cells; likewise, no changes were observed in cell morphology with respect to control, observing normal structures in the actin cytoskeleton and in the DNA structure. These results show that chitosan-based particles are a feasible and safe option for the control of pathogenic fungi of agricultural-food interest.

Translated title of the contributionPartículas de quitosano/carragenina/lisozima: Síntesis, caracterización y actividad antifúngica contra aspergillus parasiticus
Original languageEnglish
Pages (from-to)897-912
Number of pages16
JournalRevista Mexicana de Ingeniera Quimica
Volume17
Issue number3
DOIs
StatePublished - 1 Sep 2018

Bibliographical note

Publisher Copyright:
© 2018, Universidad Autonoma Metropolitana Iztapalapa. All rights reserved.

Keywords

  • Biocomposites
  • Carrageenan
  • Cytotoxicity
  • Protein release
  • Subcellular structures

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