TY - JOUR
T1 - Chymotrypsin isolation from jumbo squid (Dosidicus gigas) hepatopancreas
T2 - Partial characterization and effect on muscle collagen
AU - Marquez-Rios, Enrique
AU - Cota-Arriola, Octavio
AU - Villalba-Villalba, Ana Gloria
AU - Ezquerra-Brauer, Josafat Marina
AU - Ocaño-Higuera, Victor Manuel
AU - Lopez-Corona, Betzabe Ebenhezer
AU - Torres-Arreola, Wilfrido
N1 - Publisher Copyright:
© 2016, The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht.
PY - 2016/8/1
Y1 - 2016/8/1
N2 - Chymotrypsin was purified from jumbo squid hepatopancreas (HP) with 2.4-fold and yield 1.9%, and characterized with a molecular weight of 31 kDa, as estimated by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE). Chymotrypsin effect over collagen extracted from the mantle, fins and arms of the jumbo squid was evaluated. The enzyme exhibited the maximum activity at pH 7 and 65°C using Suc-Ala-Ala-Pro-Phe-p-nitroanilide (SAAPNA) as a substrate and it was identified using the specific inhibitors N-tosyl-L-phenylalaninechloromethyl ketone (TPCK) and phenyl methyl sulfonyl fluoride (PMSF), showing residual activities of 6% and 0%, respectively. Furthermore, high activity was observed in the pH range of 4.0 to 8.0. Purified enzyme showed a moderate in vitro activity using muscle collagen as a substrate. Although further research is needed, the results suggest that the enzyme has a potential application where acidic or slightly alkaline conditions are needed.
AB - Chymotrypsin was purified from jumbo squid hepatopancreas (HP) with 2.4-fold and yield 1.9%, and characterized with a molecular weight of 31 kDa, as estimated by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE). Chymotrypsin effect over collagen extracted from the mantle, fins and arms of the jumbo squid was evaluated. The enzyme exhibited the maximum activity at pH 7 and 65°C using Suc-Ala-Ala-Pro-Phe-p-nitroanilide (SAAPNA) as a substrate and it was identified using the specific inhibitors N-tosyl-L-phenylalaninechloromethyl ketone (TPCK) and phenyl methyl sulfonyl fluoride (PMSF), showing residual activities of 6% and 0%, respectively. Furthermore, high activity was observed in the pH range of 4.0 to 8.0. Purified enzyme showed a moderate in vitro activity using muscle collagen as a substrate. Although further research is needed, the results suggest that the enzyme has a potential application where acidic or slightly alkaline conditions are needed.
KW - Dosidicus gigas
KW - activity
KW - characterization
KW - chymotrypsin
KW - hepatopancreas
UR - http://www.scopus.com/inward/record.url?scp=84986261681&partnerID=8YFLogxK
U2 - 10.1007/s10068-016-0163-y
DO - 10.1007/s10068-016-0163-y
M3 - Artículo
SN - 1226-7708
VL - 25
SP - 1011
EP - 1016
JO - Food Science and Biotechnology
JF - Food Science and Biotechnology
IS - 4
ER -