Chymotrypsin isolation from jumbo squid (Dosidicus gigas) hepatopancreas: Partial characterization and effect on muscle collagen

Enrique Marquez-Rios, Octavio Cota-Arriola, Ana Gloria Villalba-Villalba, Josafat Marina Ezquerra-Brauer, Victor Manuel Ocaño-Higuera, Betzabe Ebenhezer Lopez-Corona, Wilfrido Torres-Arreola*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Chymotrypsin was purified from jumbo squid hepatopancreas (HP) with 2.4-fold and yield 1.9%, and characterized with a molecular weight of 31 kDa, as estimated by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE). Chymotrypsin effect over collagen extracted from the mantle, fins and arms of the jumbo squid was evaluated. The enzyme exhibited the maximum activity at pH 7 and 65°C using Suc-Ala-Ala-Pro-Phe-p-nitroanilide (SAAPNA) as a substrate and it was identified using the specific inhibitors N-tosyl-L-phenylalaninechloromethyl ketone (TPCK) and phenyl methyl sulfonyl fluoride (PMSF), showing residual activities of 6% and 0%, respectively. Furthermore, high activity was observed in the pH range of 4.0 to 8.0. Purified enzyme showed a moderate in vitro activity using muscle collagen as a substrate. Although further research is needed, the results suggest that the enzyme has a potential application where acidic or slightly alkaline conditions are needed.

Original languageEnglish
Pages (from-to)1011-1016
Number of pages6
JournalFood Science and Biotechnology
Volume25
Issue number4
DOIs
StatePublished - 1 Aug 2016

Bibliographical note

Publisher Copyright:
© 2016, The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht.

Keywords

  • Dosidicus gigas
  • activity
  • characterization
  • chymotrypsin
  • hepatopancreas

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