TY - JOUR
T1 - Combination of ultraviolet light-C and clove essential oil to inactivate Salmonella Typhimurium biofilms on stainless steel
AU - Silva-Espinoza, Brenda A.
AU - Palomares-Navarro, Julian J.
AU - Tapia-Rodriguez, Melvin R.
AU - Cruz-Valenzuela, Manuel R.
AU - González-Aguilar, Gustavo A.
AU - Silva-Campa, Erika
AU - Pedroza-Montero, Martín
AU - Almeida-Lopes, Monica
AU - Miranda, Raquel
AU - Ayala-Zavala, Jesus F.
N1 - Funding Information:
Consejo Nacional de Ciencia y Tecnolog?a, Grant/Award Number: CB-2013-01-222691
Publisher Copyright:
© 2020 Wiley Periodicals, Inc.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/6/1
Y1 - 2020/6/1
N2 - Salmonella typhimurium is able to form biofilms as a resistance mechanism against antimicrobials; therefore, it represents a problem for assuring food safety and highlights the importance of research on anti-biofilm technologies. In this study, S. typhimurium biofilms were inactivated with the combination of clove essential oil (CEO) and ultraviolet light (UV-C). The volatile composition of the CEO determined by gas chromatography showed eugenol as the major constituent (82%). A combination of CEO with UV-C achieved a complete bacterial reduction (6.8 log/cm2) on biofilms with doses of 1.2 mg/ml and 76.41 mJ/cm2, respectively. Individually, the CEO at 1.2 mg/ml caused a reduction of 1.8 log CFU/cm2 of attached bacteria cells on stainless steel, while UV-C individually used at 620.4 mJ/cm2 caused a 2.9 log CFU/cm2 reduction compared to control biofilms. In conclusion, this study demonstrated a synergistic effect of combining CEO and UV-C irradiation to inactivate biofilms of S. typhimurium.
AB - Salmonella typhimurium is able to form biofilms as a resistance mechanism against antimicrobials; therefore, it represents a problem for assuring food safety and highlights the importance of research on anti-biofilm technologies. In this study, S. typhimurium biofilms were inactivated with the combination of clove essential oil (CEO) and ultraviolet light (UV-C). The volatile composition of the CEO determined by gas chromatography showed eugenol as the major constituent (82%). A combination of CEO with UV-C achieved a complete bacterial reduction (6.8 log/cm2) on biofilms with doses of 1.2 mg/ml and 76.41 mJ/cm2, respectively. Individually, the CEO at 1.2 mg/ml caused a reduction of 1.8 log CFU/cm2 of attached bacteria cells on stainless steel, while UV-C individually used at 620.4 mJ/cm2 caused a 2.9 log CFU/cm2 reduction compared to control biofilms. In conclusion, this study demonstrated a synergistic effect of combining CEO and UV-C irradiation to inactivate biofilms of S. typhimurium.
UR - http://www.scopus.com/inward/record.url?scp=85081313660&partnerID=8YFLogxK
U2 - 10.1111/jfs.12788
DO - 10.1111/jfs.12788
M3 - Artículo
AN - SCOPUS:85081313660
SN - 0149-6085
VL - 40
JO - Journal of Food Safety
JF - Journal of Food Safety
IS - 3
M1 - e12788
ER -