Hard red winter wheat samples collected from different locations in Kansas from the 1993, 1994, 1995, and 1996 harvests were plated to determine Fusarium graminearum infection and analyzed for deoxynivalenol by thin-layer and gas chromatography. Rainfall, temperature, and cultivar were important factors affecting the severity of F. graminearum infection as well as deoxynivalenol production. The 1993 and 1995 growing seasons had high percentages of samples infected with F. graminearum and contaminated with deoxynivalenol. Averaged over the four years, cultivars 2163 and Karl had significantly higher levels of infection than did TAM 107. These widely grown cultivars were used in comparison. Northeastern Kansas had the highest levels of F. graminearum infection and deoxynivalenol contamination but also had the lowest acreage planted to hard red winter wheat.