Conductometric uric acid and urea biosensor prepared from electroconductive polyaniline-poly(n-butyl methacrylate) composites

M. M. Castillo-Ortega, D. E. Rodriguez, J. C. Encinas, M. Plascencia, F. A. Méndez-Velarde, R. Olayo

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117 Scopus citations

Abstract

Polyaniline-poly(n-butyl methacrylate) (PAn-PBMA) composite films were obtained by casting. The homogeneous films were prepared with poly(vinyl methyl ether) (PVME) and poly(vinyl ethyl ether) (PVEE) as dispersants, and the electric conductivity, mechanical properties and morphology were studied. The PAn integration to the composite is better when PVEE is used as dispersant, but the conductivity is higher when PVME is used. The composites with PVME were tested as chemical sensors for H2O2 and NH3, showing good performance. These samples were also tested as biosensor for urea and uric acid using urease and uricase enzymes, respectively. The best results were as chemical sensor for H2O2, NH3, and as biosensors for serum urea determination.

Original languageEnglish
Pages (from-to)19-25
Number of pages7
JournalSensors and Actuators, B: Chemical
Volume85
Issue number1-2
DOIs
StatePublished - 20 Jun 2002

Bibliographical note

Funding Information:
This work was supported by the Consejo Nacional de Ciencia y Tecnologia, México (Grant No. 31334-U). The authors thank M. Inoue and M. Barboza for their valuable discussions and F. Brown for his assistance in obtaining SEM images of the composites. One of the authors, J.C. Encinas, thanks Industrias Resistol S.A. for providing the financial support.

Keywords

  • Composites
  • Electroconductive polymers
  • Polyaniline
  • Urea sensor
  • Uric acid sensor

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