Conformational behavior, topographical features, and antioxidant activity of partly de-esterified arabinoxylans

Yubia De Anda-Flores, Elizabeth Carvajal-Millan*, Jaime Lizardi-Mendoza, Agustin Rascon-Chu, Judith Tanori-Cordova, Ana Luisa Martínez-López, Alexel J. Burgara-Estrella, Martin R. Pedroza-Montero

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

This study aimed to investigate the effect of arabinoxylans (AX) partial de-esterification with feruloyl esterase on the polysaccharide conformational behavior, topographical features, and antioxidant activity. After enzyme treatment, the ferulic acid (FA) content in AX was reduced from 7.30 to 5.48 µg FA/mg polysaccharide, and the molecule registered a small reduction in radius of gyration (RG), hydrodynamic radius (Rh), characteristic ratio (C∞), and persistence length (q). A slight decrease in α and a small increase in K constants in the Mark–Houwink–Sakurada equation for partially de-esterified AX (FAX) suggested a reduction in molecule structural rigidity and a more expanded coil conformation, respectively, in relation to AX. Fourier transform infrared spectroscopy spectra of AX and FAX presented a pattern characteristic for this polysaccharide. Atomic force microscopy topographic analysis of FAX showed a more regular surface without larger hollows in relation to AX. The antioxidant activity of FAX, compared to AX, was reduced by 30 and 41% using both 2,2-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS+) and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) methods, respectively. These results suggest that feruloyl esterase treatment of AX could offer a strategy to tailor AX chains conformation, morphological features, and antioxidant activity, impacting the development of advanced biomaterials for biomedical and pharmaceutical applications.

Original languageEnglish
Article number2794
JournalPolymers
Volume13
Issue number16
DOIs
StatePublished - 2 Aug 2021

Bibliographical note

Funding Information:
Funding: This research was supported by CONACYT INFR-2014-01-226082, INFR-2016-01-268666, and INFR-2018-01-293642.

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

  • Arabinoxylan
  • Ferulic acid
  • Macromolecular characteristics
  • Partial de-esterification

Fingerprint

Dive into the research topics of 'Conformational behavior, topographical features, and antioxidant activity of partly de-esterified arabinoxylans'. Together they form a unique fingerprint.

Cite this