TY - JOUR
T1 - Core-shell nanoparticles from fucoidan neoglycans
T2 - Synthesis, characterization with capability of glycomimetic ligands for Campylobacter jejuni
AU - Sandoval Larios, Giovanna
AU - Huerta Ocampo, José Ángel
AU - Sarabia Sainz, Jose Andrei
AU - García Galaz, Alfonso
AU - Guzmán-Partida, Ana María
AU - Madera Santana, Tomás Jesús
AU - Ramos-Clamont Montfort, Gabriela
N1 - Publisher Copyright:
© 2023
PY - 2023/12
Y1 - 2023/12
N2 - Campylobacteriosis produced by Campylobacter jejuni is the main cause of bacterial gastroenteritis worldwide and the binding of this pathogen to fucosylated glycoconjugates expressed on host cells is a determining factor for the infection. The aim of this work was to synthesize and characterize core-shell nanoparticles from fucoidan neoglycans as potential glycomimetic ligands for Campylobacter jejuni. Fucoidan oligosaccharides (OFuc) were obtained by mild hydrolysis of fucoidan. OFuc were separated by ultrafiltration, characterized by dynamic light scattering (DLS), infrared spectroscopy (FTIR), Fluorophore-assisted carbohydrate electrophoresis and lectin recognition. The OFuc1 were then conjugated with bovine serum albumin (BSA) by controlled glycation. The formation of fucosylated BSA (BSA-OFuc1) was confirmed by FTIR, increased surface charge, polyacrylamide gel electrophoresis, mass spectrometry and Ulex europaeus I lectin recognition. Afterwards core-shell BSA/BSA-OFuc1 nanoparticles were obtained by crosslinking. These nanoparticles presented hemispherical form with size and charge distribution of 312.1 ± 3.2 nm and -34.4 ± 0.3 mV respectively and were selectively recognized by C. jejuni by an ELISA-like lectin assay (ELLA). These results revel the potential of core-shell nanoparticles from fucoidan neoglycans as glycomimetic ligands for Campylobacter jejuni and open further opportunities to explore potential applications in the pharmaceutic industry.
AB - Campylobacteriosis produced by Campylobacter jejuni is the main cause of bacterial gastroenteritis worldwide and the binding of this pathogen to fucosylated glycoconjugates expressed on host cells is a determining factor for the infection. The aim of this work was to synthesize and characterize core-shell nanoparticles from fucoidan neoglycans as potential glycomimetic ligands for Campylobacter jejuni. Fucoidan oligosaccharides (OFuc) were obtained by mild hydrolysis of fucoidan. OFuc were separated by ultrafiltration, characterized by dynamic light scattering (DLS), infrared spectroscopy (FTIR), Fluorophore-assisted carbohydrate electrophoresis and lectin recognition. The OFuc1 were then conjugated with bovine serum albumin (BSA) by controlled glycation. The formation of fucosylated BSA (BSA-OFuc1) was confirmed by FTIR, increased surface charge, polyacrylamide gel electrophoresis, mass spectrometry and Ulex europaeus I lectin recognition. Afterwards core-shell BSA/BSA-OFuc1 nanoparticles were obtained by crosslinking. These nanoparticles presented hemispherical form with size and charge distribution of 312.1 ± 3.2 nm and -34.4 ± 0.3 mV respectively and were selectively recognized by C. jejuni by an ELISA-like lectin assay (ELLA). These results revel the potential of core-shell nanoparticles from fucoidan neoglycans as glycomimetic ligands for Campylobacter jejuni and open further opportunities to explore potential applications in the pharmaceutic industry.
KW - Biorecognition
KW - Campylobacter jejuni
KW - Core-shell nanoparticles
KW - Fucoidan neoglycans
KW - Glycomimetic ligands
KW - Maillard fucosylation
UR - http://www.scopus.com/inward/record.url?scp=85175635304&partnerID=8YFLogxK
U2 - 10.1016/j.carpta.2023.100384
DO - 10.1016/j.carpta.2023.100384
M3 - Artículo
AN - SCOPUS:85175635304
SN - 2666-8939
VL - 6
JO - Carbohydrate Polymer Technologies and Applications
JF - Carbohydrate Polymer Technologies and Applications
M1 - 100384
ER -