TY - JOUR
T1 - Detection and partial characterization of antimutagenic compounds from white shrimp (Litopenaeus vannamei) cephalothorax
AU - Trujillo-Ruiz, Héctor Enrique
AU - López-Saiz, Carmen María
AU - García-Alegría, Alejandro Monserrat
AU - Osuna-Ruiz, Idalia
AU - Santacruz-Ortega, Hisila Del Carmen
AU - Meza-Montenegro, María Mercedes
AU - Maldonado-Escalante, Juan Francisco
AU - Cruz-Ramirez, Susana Gabriela
AU - Burgos Hernández, Armando
N1 - Publisher Copyright:
© 2024 The Author(s). Published with license by Taylor & Francis Group, LLC.
PY - 2024
Y1 - 2024
N2 - Mutations are the result of effects produced by mutagenic agents, which may be responsible for the generation of chronic-degenerative diseases such as cancer. The aim of this work was to detect and characterize compounds with antimutagenic activity in white shrimp (Litopenaeus vannamei) cephalothorax. A serial extraction was carried out where different organic solvents, and its biological activity was evaluated. The antioxidant capacity was evaluated by DPPH and ABTS assay, where the methanol and water-soluble extracts had the highest antioxidant capacity. Antimutagenicity was evaluated by the Ames test, where the methanol-soluble extract showed the highest antimutagenic activity. The methanolic extract was fractionated by open column chromatography, the fractions obtained were determined antioxidant and antimutagenic activity and the ability to protect DNA from oxidative damage. Fraction H27 provided DNA the highest protection against the damage caused by H2O2. Partial chemical-characterization of fraction H27 suggests the presence of aromatic, hydroxylated, unsaturated compounds.
AB - Mutations are the result of effects produced by mutagenic agents, which may be responsible for the generation of chronic-degenerative diseases such as cancer. The aim of this work was to detect and characterize compounds with antimutagenic activity in white shrimp (Litopenaeus vannamei) cephalothorax. A serial extraction was carried out where different organic solvents, and its biological activity was evaluated. The antioxidant capacity was evaluated by DPPH and ABTS assay, where the methanol and water-soluble extracts had the highest antioxidant capacity. Antimutagenicity was evaluated by the Ames test, where the methanol-soluble extract showed the highest antimutagenic activity. The methanolic extract was fractionated by open column chromatography, the fractions obtained were determined antioxidant and antimutagenic activity and the ability to protect DNA from oxidative damage. Fraction H27 provided DNA the highest protection against the damage caused by H2O2. Partial chemical-characterization of fraction H27 suggests the presence of aromatic, hydroxylated, unsaturated compounds.
KW - antimutagenic
KW - Antioxidant
KW - bioactivity
KW - by-product
KW - shrimp
UR - http://www.scopus.com/inward/record.url?scp=85185841859&partnerID=8YFLogxK
U2 - 10.1080/19476337.2024.2315990
DO - 10.1080/19476337.2024.2315990
M3 - Artículo
AN - SCOPUS:85185841859
SN - 1947-6337
VL - 22
JO - CYTA - Journal of Food
JF - CYTA - Journal of Food
IS - 1
M1 - 2315990
ER -