Detection of immunological reaction with the attenuated total reflection (ATR) technique

E. Rodriguez, L.E. Regalado

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

The sensitivity of the Attenuated Total Reflection (ATR) technique to surface modifications allows the study of biological or medical phenomena where solutions seggregate to form a layer on the walls of the sample holder. An aluminum thin-film is deposited at the bottom of the container where the reaction takes place and is attached to a prism. During the reaction a layer is adsorbed on the aluminum film. The study of the total reflectivity in this system when the incoming light enters through the prism shows a resonant absorption peak that shifts towards larger angles of incidence when monochromatic light is used. This shift is related to the optical properties and the thickness of the adsorbed layers and may be quantified. We present here the study of Antigen-Antibody reactions with the aid of this technique.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
Subtitle of host publicationOptics for Science and New Technology
Pages1045-1046
Number of pages2
EditionPART 2
StatePublished - 1996
Event17th Congress of the International Commission for Optics: Optics for Science and New Technology, ICO 1996 - Taejon, Korea, Republic of
Duration: 19 Aug 199623 Aug 1996

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
NumberPART 2
Volume2778
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

Conference17th Congress of the International Commission for Optics: Optics for Science and New Technology, ICO 1996
Country/TerritoryKorea, Republic of
CityTaejon
Period19/08/9623/08/96

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