Development and characterization of a monoclonal antibody against porcine CD205

Lilian Flores-Mendoza; Carlos Velazquez; Jocelyn Bray; Leo Njongmeta; Waithaka Mwangi; Jesús Hernández

doi:10.1016/j.vetimm.2012.01.011

Development and characterization of a monoclonal antibody against porcine CD205

Lilian Flores-Mendoza, Carlos Velazquez, Jocelyn Bray, Leo Njongmeta, Waithaka Mwangi, Jesús Hernández^*

^*Corresponding author for this work

Departamento de Ciencias Químico-Biológicas

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The aim of this work was to develop mAbs against porcine CD205 and to conduct a comparative analysis of the CD205 protein expression on lymphoid tissues, monocyte-derived dendritic cells (DCs) and DCs isolated from the porcine skin. To conduct this study, we generated a monoclonal antibody, designated 1.F6F6, against the C-type lectin-like domain-5 of the porcine CD205 and showed that it recognizes a protein band of ∼200. kDa by Western Blot analysis in mesenteric lymph nodes cells. Flow cytometric analysis showed that the mAb 1.F6F6 recognized 28.5%, 28.1% and 34.1% of cells from tonsil, inguinal and mesenteric lymph nodes, respectively, and 6% of cells from thymus. Analysis of monocyte-derived DCs showed that approximately 20% were positive and activation of the cells with LPS increased the positive population to 36%. Analysis of DCs isolated from the porcine skin showed that approximately 70% of the cell population expressed the CD205 receptor. The development of a monoclonal antibody capable of recognizing the CD205 receptor in swine opens up possibilities of applying new strategies for enhancing vaccine efficacy by using the anti-CD205 antibody for DC antigen-targeting to enhance priming of immune responses.

Original language	English
Pages (from-to)	74-80
Number of pages	7
Journal	Veterinary Immunology and Immunopathology
Volume	146
Issue number	1
DOIs	https://doi.org/10.1016/j.vetimm.2012.01.011
State	Published - 15 Mar 2012

Bibliographical note

Funding Information:
This work was supported by SEP-CONACyT grant 82850 to Dr. J. Hernandez and TAMU-CONACyT CRG-2007 to Dr. W. Mwangi and Dr. J. Hernandez. We are grateful to Mónica Reséndiz for their technical support.

Keywords

C-type lectin receptors
CD205
Dendritic cells
Porcine

Access to Document

10.1016/j.vetimm.2012.01.011

Cite this

@article{6a093f2b86af4968b48457f290976209,

title = "Development and characterization of a monoclonal antibody against porcine CD205",

abstract = "The aim of this work was to develop mAbs against porcine CD205 and to conduct a comparative analysis of the CD205 protein expression on lymphoid tissues, monocyte-derived dendritic cells (DCs) and DCs isolated from the porcine skin. To conduct this study, we generated a monoclonal antibody, designated 1.F6F6, against the C-type lectin-like domain-5 of the porcine CD205 and showed that it recognizes a protein band of ∼200. kDa by Western Blot analysis in mesenteric lymph nodes cells. Flow cytometric analysis showed that the mAb 1.F6F6 recognized 28.5%, 28.1% and 34.1% of cells from tonsil, inguinal and mesenteric lymph nodes, respectively, and 6% of cells from thymus. Analysis of monocyte-derived DCs showed that approximately 20% were positive and activation of the cells with LPS increased the positive population to 36%. Analysis of DCs isolated from the porcine skin showed that approximately 70% of the cell population expressed the CD205 receptor. The development of a monoclonal antibody capable of recognizing the CD205 receptor in swine opens up possibilities of applying new strategies for enhancing vaccine efficacy by using the anti-CD205 antibody for DC antigen-targeting to enhance priming of immune responses.",

keywords = "C-type lectin receptors, CD205, Dendritic cells, Porcine",

author = "Lilian Flores-Mendoza and Carlos Velazquez and Jocelyn Bray and Leo Njongmeta and Waithaka Mwangi and Jes{\'u}s Hern{\'a}ndez",

note = "Funding Information: This work was supported by SEP-CONACyT grant 82850 to Dr. J. Hernandez and TAMU-CONACyT CRG-2007 to Dr. W. Mwangi and Dr. J. Hernandez. We are grateful to M{\'o}nica Res{\'e}ndiz for their technical support.",

year = "2012",

month = mar,

day = "15",

doi = "10.1016/j.vetimm.2012.01.011",

language = "Ingl{\'e}s",

volume = "146",

pages = "74--80",

journal = "Veterinary Immunology and Immunopathology",

issn = "0165-2427",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Development and characterization of a monoclonal antibody against porcine CD205

AU - Flores-Mendoza, Lilian

AU - Velazquez, Carlos

AU - Bray, Jocelyn

AU - Njongmeta, Leo

AU - Mwangi, Waithaka

AU - Hernández, Jesús

N1 - Funding Information: This work was supported by SEP-CONACyT grant 82850 to Dr. J. Hernandez and TAMU-CONACyT CRG-2007 to Dr. W. Mwangi and Dr. J. Hernandez. We are grateful to Mónica Reséndiz for their technical support.

PY - 2012/3/15

Y1 - 2012/3/15

N2 - The aim of this work was to develop mAbs against porcine CD205 and to conduct a comparative analysis of the CD205 protein expression on lymphoid tissues, monocyte-derived dendritic cells (DCs) and DCs isolated from the porcine skin. To conduct this study, we generated a monoclonal antibody, designated 1.F6F6, against the C-type lectin-like domain-5 of the porcine CD205 and showed that it recognizes a protein band of ∼200. kDa by Western Blot analysis in mesenteric lymph nodes cells. Flow cytometric analysis showed that the mAb 1.F6F6 recognized 28.5%, 28.1% and 34.1% of cells from tonsil, inguinal and mesenteric lymph nodes, respectively, and 6% of cells from thymus. Analysis of monocyte-derived DCs showed that approximately 20% were positive and activation of the cells with LPS increased the positive population to 36%. Analysis of DCs isolated from the porcine skin showed that approximately 70% of the cell population expressed the CD205 receptor. The development of a monoclonal antibody capable of recognizing the CD205 receptor in swine opens up possibilities of applying new strategies for enhancing vaccine efficacy by using the anti-CD205 antibody for DC antigen-targeting to enhance priming of immune responses.

AB - The aim of this work was to develop mAbs against porcine CD205 and to conduct a comparative analysis of the CD205 protein expression on lymphoid tissues, monocyte-derived dendritic cells (DCs) and DCs isolated from the porcine skin. To conduct this study, we generated a monoclonal antibody, designated 1.F6F6, against the C-type lectin-like domain-5 of the porcine CD205 and showed that it recognizes a protein band of ∼200. kDa by Western Blot analysis in mesenteric lymph nodes cells. Flow cytometric analysis showed that the mAb 1.F6F6 recognized 28.5%, 28.1% and 34.1% of cells from tonsil, inguinal and mesenteric lymph nodes, respectively, and 6% of cells from thymus. Analysis of monocyte-derived DCs showed that approximately 20% were positive and activation of the cells with LPS increased the positive population to 36%. Analysis of DCs isolated from the porcine skin showed that approximately 70% of the cell population expressed the CD205 receptor. The development of a monoclonal antibody capable of recognizing the CD205 receptor in swine opens up possibilities of applying new strategies for enhancing vaccine efficacy by using the anti-CD205 antibody for DC antigen-targeting to enhance priming of immune responses.

KW - C-type lectin receptors

KW - CD205

KW - Dendritic cells

KW - Porcine

UR - http://www.scopus.com/inward/record.url?scp=84857801431&partnerID=8YFLogxK

U2 - 10.1016/j.vetimm.2012.01.011

DO - 10.1016/j.vetimm.2012.01.011

M3 - Artículo

C2 - 22348805

SN - 0165-2427

VL - 146

SP - 74

EP - 80

JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

IS - 1

ER -

Development and characterization of a monoclonal antibody against porcine CD205

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this