Differential Response of BEAS-2B and H-441 Cells to Methylene Blue Photoactivation

Rosalva Josefina Rodríguez-Córdova, Cindy Alejandra Gutiérrez-Valenzuela, Pasano Bojang, Reynaldo Esquivel, Pedro Hernández, Kenneth S Ramos, Roberto Guzmán-Zamudio, Armando Lucero-Acuña

Research output: Contribution to journalArticleResearchpeer-review

Abstract

BACKGROUND/AIM: Cancer incidence and mortalities are growing worldwide, therefore research and development of more effective and less invasive treatments, such as photodynamic therapy, are needed. Herein, we investigated the methylene blue (MB) photoactivation effects in lung epithelial cells (BEAS-2B) and lung adenocarcinoma cells (H-441).

MATERIALS AND METHODS: The reactive oxygen species (ROS) produced by the laser photoactivation of MB in aqueous solutions and cell cultures were measured with probes, and the cell viability was evaluated with a colorimetric assay.

RESULTS: MB up to 31.26 μM did not induce detectable effects in BEAS-2B cells. However, H-441 cells presented adverse effects below that concentration in the same range of fluencies studied. These results are in concordance with the ROS production in H-441 cells, while in BEAS-2B cells the production of ROS was less significant compared to the control.

CONCLUSION: Photoactivation of MB at concentrations below 31.26 μM could be used for the selective treatment of H-441 cells over non-cancer cells.

Original languageEnglish
Pages (from-to)3739-3744
Number of pages6
JournalAnticancer Research
Volume39
Issue number7
DOIs
StatePublished - Jul 2019
Externally publishedYes

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Methylene Blue
Reactive Oxygen Species
Photochemotherapy
Cell Survival
Lasers
Cell Culture Techniques
Epithelial Cells
Lung
Mortality
Incidence
Therapeutics
Research
Neoplasms

Cite this

Rodríguez-Córdova, R. J., Gutiérrez-Valenzuela, C. A., Bojang, P., Esquivel, R., Hernández, P., Ramos, K. S., ... Lucero-Acuña, A. (2019). Differential Response of BEAS-2B and H-441 Cells to Methylene Blue Photoactivation. Anticancer Research, 39(7), 3739-3744. https://doi.org/10.21873/anticanres.13522
Rodríguez-Córdova, Rosalva Josefina ; Gutiérrez-Valenzuela, Cindy Alejandra ; Bojang, Pasano ; Esquivel, Reynaldo ; Hernández, Pedro ; Ramos, Kenneth S ; Guzmán-Zamudio, Roberto ; Lucero-Acuña, Armando. / Differential Response of BEAS-2B and H-441 Cells to Methylene Blue Photoactivation. In: Anticancer Research. 2019 ; Vol. 39, No. 7. pp. 3739-3744.
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abstract = "BACKGROUND/AIM: Cancer incidence and mortalities are growing worldwide, therefore research and development of more effective and less invasive treatments, such as photodynamic therapy, are needed. Herein, we investigated the methylene blue (MB) photoactivation effects in lung epithelial cells (BEAS-2B) and lung adenocarcinoma cells (H-441).MATERIALS AND METHODS: The reactive oxygen species (ROS) produced by the laser photoactivation of MB in aqueous solutions and cell cultures were measured with probes, and the cell viability was evaluated with a colorimetric assay.RESULTS: MB up to 31.26 μM did not induce detectable effects in BEAS-2B cells. However, H-441 cells presented adverse effects below that concentration in the same range of fluencies studied. These results are in concordance with the ROS production in H-441 cells, while in BEAS-2B cells the production of ROS was less significant compared to the control.CONCLUSION: Photoactivation of MB at concentrations below 31.26 μM could be used for the selective treatment of H-441 cells over non-cancer cells.",
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year = "2019",
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Rodríguez-Córdova, RJ, Gutiérrez-Valenzuela, CA, Bojang, P, Esquivel, R, Hernández, P, Ramos, KS, Guzmán-Zamudio, R & Lucero-Acuña, A 2019, 'Differential Response of BEAS-2B and H-441 Cells to Methylene Blue Photoactivation', Anticancer Research, vol. 39, no. 7, pp. 3739-3744. https://doi.org/10.21873/anticanres.13522

Differential Response of BEAS-2B and H-441 Cells to Methylene Blue Photoactivation. / Rodríguez-Córdova, Rosalva Josefina; Gutiérrez-Valenzuela, Cindy Alejandra; Bojang, Pasano; Esquivel, Reynaldo; Hernández, Pedro; Ramos, Kenneth S; Guzmán-Zamudio, Roberto; Lucero-Acuña, Armando.

In: Anticancer Research, Vol. 39, No. 7, 07.2019, p. 3739-3744.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Differential Response of BEAS-2B and H-441 Cells to Methylene Blue Photoactivation

AU - Rodríguez-Córdova, Rosalva Josefina

AU - Gutiérrez-Valenzuela, Cindy Alejandra

AU - Bojang, Pasano

AU - Esquivel, Reynaldo

AU - Hernández, Pedro

AU - Ramos, Kenneth S

AU - Guzmán-Zamudio, Roberto

AU - Lucero-Acuña, Armando

N1 - Copyright© 2019, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

PY - 2019/7

Y1 - 2019/7

N2 - BACKGROUND/AIM: Cancer incidence and mortalities are growing worldwide, therefore research and development of more effective and less invasive treatments, such as photodynamic therapy, are needed. Herein, we investigated the methylene blue (MB) photoactivation effects in lung epithelial cells (BEAS-2B) and lung adenocarcinoma cells (H-441).MATERIALS AND METHODS: The reactive oxygen species (ROS) produced by the laser photoactivation of MB in aqueous solutions and cell cultures were measured with probes, and the cell viability was evaluated with a colorimetric assay.RESULTS: MB up to 31.26 μM did not induce detectable effects in BEAS-2B cells. However, H-441 cells presented adverse effects below that concentration in the same range of fluencies studied. These results are in concordance with the ROS production in H-441 cells, while in BEAS-2B cells the production of ROS was less significant compared to the control.CONCLUSION: Photoactivation of MB at concentrations below 31.26 μM could be used for the selective treatment of H-441 cells over non-cancer cells.

AB - BACKGROUND/AIM: Cancer incidence and mortalities are growing worldwide, therefore research and development of more effective and less invasive treatments, such as photodynamic therapy, are needed. Herein, we investigated the methylene blue (MB) photoactivation effects in lung epithelial cells (BEAS-2B) and lung adenocarcinoma cells (H-441).MATERIALS AND METHODS: The reactive oxygen species (ROS) produced by the laser photoactivation of MB in aqueous solutions and cell cultures were measured with probes, and the cell viability was evaluated with a colorimetric assay.RESULTS: MB up to 31.26 μM did not induce detectable effects in BEAS-2B cells. However, H-441 cells presented adverse effects below that concentration in the same range of fluencies studied. These results are in concordance with the ROS production in H-441 cells, while in BEAS-2B cells the production of ROS was less significant compared to the control.CONCLUSION: Photoactivation of MB at concentrations below 31.26 μM could be used for the selective treatment of H-441 cells over non-cancer cells.

U2 - 10.21873/anticanres.13522

DO - 10.21873/anticanres.13522

M3 - Artículo

VL - 39

SP - 3739

EP - 3744

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 7

ER -

Rodríguez-Córdova RJ, Gutiérrez-Valenzuela CA, Bojang P, Esquivel R, Hernández P, Ramos KS et al. Differential Response of BEAS-2B and H-441 Cells to Methylene Blue Photoactivation. Anticancer Research. 2019 Jul;39(7):3739-3744. https://doi.org/10.21873/anticanres.13522