Abstract
BACKGROUND/AIM: Cancer incidence and mortalities are growing worldwide, therefore research and development of more effective and less invasive treatments, such as photodynamic therapy, are needed. Herein, we investigated the methylene blue (MB) photoactivation effects in lung epithelial cells (BEAS-2B) and lung adenocarcinoma cells (H-441).
MATERIALS AND METHODS: The reactive oxygen species (ROS) produced by the laser photoactivation of MB in aqueous solutions and cell cultures were measured with probes, and the cell viability was evaluated with a colorimetric assay.
RESULTS: MB up to 31.26 μM did not induce detectable effects in BEAS-2B cells. However, H-441 cells presented adverse effects below that concentration in the same range of fluencies studied. These results are in concordance with the ROS production in H-441 cells, while in BEAS-2B cells the production of ROS was less significant compared to the control.
CONCLUSION: Photoactivation of MB at concentrations below 31.26 μM could be used for the selective treatment of H-441 cells over non-cancer cells.
| Original language | English |
|---|---|
| Pages (from-to) | 3739-3744 |
| Number of pages | 6 |
| Journal | Anticancer Research |
| Volume | 39 |
| Issue number | 7 |
| DOIs | |
| State | Published - Jul 2019 |
| Externally published | Yes |
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Differential Response of BEAS-2B and H-441 Cells to Methylene Blue Photoactivation. / Rodríguez-Córdova, Rosalva Josefina; Gutiérrez-Valenzuela, Cindy Alejandra; Bojang, Pasano; Esquivel, Reynaldo; Hernández, Pedro; Ramos, Kenneth S; Guzmán-Zamudio, Roberto; Lucero-Acuña, Armando.
In: Anticancer Research, Vol. 39, No. 7, 07.2019, p. 3739-3744.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Differential Response of BEAS-2B and H-441 Cells to Methylene Blue Photoactivation
AU - Rodríguez-Córdova, Rosalva Josefina
AU - Gutiérrez-Valenzuela, Cindy Alejandra
AU - Bojang, Pasano
AU - Esquivel, Reynaldo
AU - Hernández, Pedro
AU - Ramos, Kenneth S
AU - Guzmán-Zamudio, Roberto
AU - Lucero-Acuña, Armando
N1 - Copyright© 2019, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.
PY - 2019/7
Y1 - 2019/7
N2 - BACKGROUND/AIM: Cancer incidence and mortalities are growing worldwide, therefore research and development of more effective and less invasive treatments, such as photodynamic therapy, are needed. Herein, we investigated the methylene blue (MB) photoactivation effects in lung epithelial cells (BEAS-2B) and lung adenocarcinoma cells (H-441).MATERIALS AND METHODS: The reactive oxygen species (ROS) produced by the laser photoactivation of MB in aqueous solutions and cell cultures were measured with probes, and the cell viability was evaluated with a colorimetric assay.RESULTS: MB up to 31.26 μM did not induce detectable effects in BEAS-2B cells. However, H-441 cells presented adverse effects below that concentration in the same range of fluencies studied. These results are in concordance with the ROS production in H-441 cells, while in BEAS-2B cells the production of ROS was less significant compared to the control.CONCLUSION: Photoactivation of MB at concentrations below 31.26 μM could be used for the selective treatment of H-441 cells over non-cancer cells.
AB - BACKGROUND/AIM: Cancer incidence and mortalities are growing worldwide, therefore research and development of more effective and less invasive treatments, such as photodynamic therapy, are needed. Herein, we investigated the methylene blue (MB) photoactivation effects in lung epithelial cells (BEAS-2B) and lung adenocarcinoma cells (H-441).MATERIALS AND METHODS: The reactive oxygen species (ROS) produced by the laser photoactivation of MB in aqueous solutions and cell cultures were measured with probes, and the cell viability was evaluated with a colorimetric assay.RESULTS: MB up to 31.26 μM did not induce detectable effects in BEAS-2B cells. However, H-441 cells presented adverse effects below that concentration in the same range of fluencies studied. These results are in concordance with the ROS production in H-441 cells, while in BEAS-2B cells the production of ROS was less significant compared to the control.CONCLUSION: Photoactivation of MB at concentrations below 31.26 μM could be used for the selective treatment of H-441 cells over non-cancer cells.
U2 - 10.21873/anticanres.13522
DO - 10.21873/anticanres.13522
M3 - Artículo
VL - 39
SP - 3739
EP - 3744
JO - Anticancer Research
JF - Anticancer Research
SN - 0250-7005
IS - 7
ER -