TY - JOUR
T1 - Drug resistance phenotypes and genotypes in Mexico in representative gram-negative species
T2 - Results from the infivar network
AU - Garza-González, Elvira
AU - Bocanegra-Ibarias, Paola
AU - Bobadilla-Del-Valle, Miriam
AU - Alfredo Ponce-De-León-Garduño, Luis
AU - Esteban-Kenel, Verónica
AU - Silva-Sánchez, Jesus
AU - Garza-Ramos, Ulises
AU - Barrios-Camacho, Humberto
AU - López-Jácome, Luis Esaú
AU - Colin-Castro, Claudia A.
AU - Franco-Cendejas, Rafael
AU - Flores-Treviño, Samantha
AU - Morfín-Otero, Rayo
AU - Rojas-Larios, Fabian
AU - Mena-Ramírez, Juan Pablo
AU - Fong-Camargo, María Guadalupe
AU - Morales-De-la-Peña, Cecilia Teresita
AU - García-Mendoza, Lourdes
AU - Choy-Chang, Elena Victoria
AU - Aviles-Benitez, Laura Karina
AU - Feliciano-Guzmán, José Manuel
AU - López-Gutiérrez, Eduardo
AU - Gil-Veloz, Mariana
AU - Barajas-Magallón, Juan Manuel
AU - Aguirre-Burciaga, Efren
AU - López-Moreno, Laura Isabel
AU - Martínez-Villarreal, Rebeca Thelma
AU - Canizales-Oviedo, Jorge Luis
AU - Cetina-Umaña, Carlos Miguel
AU - Romero-Romero, Daniel
AU - Bello-Pazos, Fidencio David
AU - Barlandas-Rendón, Nicolás Rogelio Eric
AU - Maldonado-Anicacio, Joyarib Yanelli
AU - Bolado-Martínez, Enrique
AU - Galindo-Méndez, Mario
AU - Perez-Vicelis, Talia
AU - Alavez-Ramírez, Norma
AU - Méndez-Sotelo, Braulio J.
AU - Cabriales-Zavala, Juan Francisco
AU - Nava-Pacheco, Yirla Citlali
AU - Moreno-Méndez, Martha Irene
AU - García-Romo, Ricardo
AU - Silva-Gamiño, Aldo Rafael
AU - Avalos-Aguilera, Ana María
AU - Santiago-Calderón, María Asunción
AU - López-García, Maribel
AU - del Consuelo Velázquez-Acosta, María
AU - Cobos-Canul, Dulce Isabel
AU - del Rosario Vázquez-Larios, María
AU - Ortiz-Porcayo, Ana Elizabeth
AU - Guerrero-Núñez, Arely Elizabeth
AU - Valero-Guzmán, Jazmín
AU - Rosales-García, Alina Aracely
AU - Ostos-Cantú, Heidy Leticia
AU - Camacho-Ortiz, Adrián
N1 - Publisher Copyright:
Copyright: © 2021 Garza-González et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2021/3
Y1 - 2021/3
N2 - Aim This report presents phenotypic and genetic data on the prevalence and characteristics of extended-spectrum β-lactamases (ESBLs) and representative carbapenemases-producing Gram-negative species in Mexico. Material and methods A total of 52 centers participated, 43 hospital-based laboratories and 9 external laboratories. The distribution of antimicrobial resistance data for Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae complex, Acinetobacter baumannii complex, and Pseudomonas aeruginosa in selected clinical specimens from January 1 to March 31, 2020 was analyzed using the WHONET 5.6 platform. The following clinical isolates recovered from selected specimens were included: carbapenem-resistant Enterobacteriaceae, ESBL or carbapenem-resistant E. coli, and K. pneumoniae, carbapenem-resistant A. baumannii complex, and P. aeruginosa. Strains were genotyped to detect ESBL and/or carbapenemase-encoding genes. Results Among blood isolates, A. baumannii complex showed more than 68% resistance for all antibiotics tested, and among Enterobacteria, E. cloacae complex showed higher resistance to carbapenems. A. baumannii complex showed a higher resistance pattern for respiratory specimens, with only amikacin having a resistance lower than 70%. Among K. pneumoniae isolates, blaTEM, blaSHV, and blaCTX were detected in 68.79%, 72.3%, and 91.9% of isolates, respectively. Among E. coli isolates, blaTEM, blaSHV, and blaCTX were detected in 20.8%, 4.53%, and 85.7% isolates, respectively. For both species, the most frequent genotype was blaCTX-M-15. Among Enterobacteriaceae, the most frequently detected carbapenemase-encoding gene was blaNDM-1 (81.5%), followed by blaOXA-232 (14.8%) and blaoxa-181(7.4%), in A. baumannii was blaOXA-24 (76%) and in P. aeruginosa, was blaIMP (25.3%), followed by blaGES and blaVIM (13.1% each). Conclusion Our study reports that NDM-1 is the most frequent carbapenemase-encoding gene in Mexico in Enterobacteriaceae with the circulation of the oxacillinase genes 181 and 232. KPC, in contrast to other countries in Latin America and the USA, is a rare occurrence. Additionally, a high circulation of ESBL blaCTX-M-15 exists in both E. coli and K. pneumoniae.
AB - Aim This report presents phenotypic and genetic data on the prevalence and characteristics of extended-spectrum β-lactamases (ESBLs) and representative carbapenemases-producing Gram-negative species in Mexico. Material and methods A total of 52 centers participated, 43 hospital-based laboratories and 9 external laboratories. The distribution of antimicrobial resistance data for Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae complex, Acinetobacter baumannii complex, and Pseudomonas aeruginosa in selected clinical specimens from January 1 to March 31, 2020 was analyzed using the WHONET 5.6 platform. The following clinical isolates recovered from selected specimens were included: carbapenem-resistant Enterobacteriaceae, ESBL or carbapenem-resistant E. coli, and K. pneumoniae, carbapenem-resistant A. baumannii complex, and P. aeruginosa. Strains were genotyped to detect ESBL and/or carbapenemase-encoding genes. Results Among blood isolates, A. baumannii complex showed more than 68% resistance for all antibiotics tested, and among Enterobacteria, E. cloacae complex showed higher resistance to carbapenems. A. baumannii complex showed a higher resistance pattern for respiratory specimens, with only amikacin having a resistance lower than 70%. Among K. pneumoniae isolates, blaTEM, blaSHV, and blaCTX were detected in 68.79%, 72.3%, and 91.9% of isolates, respectively. Among E. coli isolates, blaTEM, blaSHV, and blaCTX were detected in 20.8%, 4.53%, and 85.7% isolates, respectively. For both species, the most frequent genotype was blaCTX-M-15. Among Enterobacteriaceae, the most frequently detected carbapenemase-encoding gene was blaNDM-1 (81.5%), followed by blaOXA-232 (14.8%) and blaoxa-181(7.4%), in A. baumannii was blaOXA-24 (76%) and in P. aeruginosa, was blaIMP (25.3%), followed by blaGES and blaVIM (13.1% each). Conclusion Our study reports that NDM-1 is the most frequent carbapenemase-encoding gene in Mexico in Enterobacteriaceae with the circulation of the oxacillinase genes 181 and 232. KPC, in contrast to other countries in Latin America and the USA, is a rare occurrence. Additionally, a high circulation of ESBL blaCTX-M-15 exists in both E. coli and K. pneumoniae.
UR - http://www.scopus.com/inward/record.url?scp=85102753744&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0248614
DO - 10.1371/journal.pone.0248614
M3 - Artículo
C2 - 33730101
AN - SCOPUS:85102753744
SN - 1932-6203
VL - 16
JO - PLoS ONE
JF - PLoS ONE
IS - 3 March
M1 - e0248614
ER -