Drug resistance phenotypes and genotypes in Mexico in representative gram-negative species: Results from the infivar network

Elvira Garza-González, Paola Bocanegra-Ibarias, Miriam Bobadilla-Del-Valle, Luis Alfredo Ponce-De-León-Garduño, Verónica Esteban-Kenel, Jesus Silva-Sánchez, Ulises Garza-Ramos, Humberto Barrios-Camacho, Luis Esaú López-Jácome, Claudia A. Colin-Castro, Rafael Franco-Cendejas, Samantha Flores-Treviño, Rayo Morfín-Otero, Fabian Rojas-Larios, Juan Pablo Mena-Ramírez, María Guadalupe Fong-Camargo, Cecilia Teresita Morales-De-la-Peña, Lourdes García-Mendoza, Elena Victoria Choy-Chang, Laura Karina Aviles-BenitezJosé Manuel Feliciano-Guzmán, Eduardo López-Gutiérrez, Mariana Gil-Veloz, Juan Manuel Barajas-Magallón, Efren Aguirre-Burciaga, Laura Isabel López-Moreno, Rebeca Thelma Martínez-Villarreal, Jorge Luis Canizales-Oviedo, Carlos Miguel Cetina-Umaña, Daniel Romero-Romero, Fidencio David Bello-Pazos, Nicolás Rogelio Eric Barlandas-Rendón, Joyarib Yanelli Maldonado-Anicacio, Enrique Bolado-Martínez, Mario Galindo-Méndez, Talia Perez-Vicelis, Norma Alavez-Ramírez, Braulio J. Méndez-Sotelo, Juan Francisco Cabriales-Zavala, Yirla Citlali Nava-Pacheco, Martha Irene Moreno-Méndez, Ricardo García-Romo, Aldo Rafael Silva-Gamiño, Ana María Avalos-Aguilera, María Asunción Santiago-Calderón, Maribel López-García, María del Consuelo Velázquez-Acosta, Dulce Isabel Cobos-Canul, María del Rosario Vázquez-Larios, Ana Elizabeth Ortiz-Porcayo, Arely Elizabeth Guerrero-Núñez, Jazmín Valero-Guzmán, Alina Aracely Rosales-García, Heidy Leticia Ostos-Cantú, Adrián Camacho-Ortiz*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

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Abstract

Aim This report presents phenotypic and genetic data on the prevalence and characteristics of extended-spectrum β-lactamases (ESBLs) and representative carbapenemases-producing Gram-negative species in Mexico. Material and methods A total of 52 centers participated, 43 hospital-based laboratories and 9 external laboratories. The distribution of antimicrobial resistance data for Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae complex, Acinetobacter baumannii complex, and Pseudomonas aeruginosa in selected clinical specimens from January 1 to March 31, 2020 was analyzed using the WHONET 5.6 platform. The following clinical isolates recovered from selected specimens were included: carbapenem-resistant Enterobacteriaceae, ESBL or carbapenem-resistant E. coli, and K. pneumoniae, carbapenem-resistant A. baumannii complex, and P. aeruginosa. Strains were genotyped to detect ESBL and/or carbapenemase-encoding genes. Results Among blood isolates, A. baumannii complex showed more than 68% resistance for all antibiotics tested, and among Enterobacteria, E. cloacae complex showed higher resistance to carbapenems. A. baumannii complex showed a higher resistance pattern for respiratory specimens, with only amikacin having a resistance lower than 70%. Among K. pneumoniae isolates, blaTEM, blaSHV, and blaCTX were detected in 68.79%, 72.3%, and 91.9% of isolates, respectively. Among E. coli isolates, blaTEM, blaSHV, and blaCTX were detected in 20.8%, 4.53%, and 85.7% isolates, respectively. For both species, the most frequent genotype was blaCTX-M-15. Among Enterobacteriaceae, the most frequently detected carbapenemase-encoding gene was blaNDM-1 (81.5%), followed by blaOXA-232 (14.8%) and blaoxa-181(7.4%), in A. baumannii was blaOXA-24 (76%) and in P. aeruginosa, was blaIMP (25.3%), followed by blaGES and blaVIM (13.1% each). Conclusion Our study reports that NDM-1 is the most frequent carbapenemase-encoding gene in Mexico in Enterobacteriaceae with the circulation of the oxacillinase genes 181 and 232. KPC, in contrast to other countries in Latin America and the USA, is a rare occurrence. Additionally, a high circulation of ESBL blaCTX-M-15 exists in both E. coli and K. pneumoniae.

Original languageEnglish
Article numbere0248614
JournalPLoS ONE
Volume16
Issue number3 March
DOIs
StatePublished - Mar 2021

Bibliographical note

Publisher Copyright:
Copyright: © 2021 Garza-González et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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