TY - JOUR
T1 - Effect of exogenous vitamin E on proliferation and cytokine production in peripheral blood mononuclear cells from patients with tuberculosis
AU - Hernández, Jesús
AU - Garibay-escobar, Adriana
AU - Mendoza-mendoza, Andrés
AU - Pinelli-saavedra, Araceli
AU - Valenzuela, Olivia
PY - 2008/2
Y1 - 2008/2
N2 - Micronutrient deficiencies are frequently associated with tuberculosis (TB) worldwide. We tested the effect of exogenous vitamin E on proliferation and cytokine production of peripheral blood mononuclear cells (PBMC) from TB patients and healthy purified protein derivative (PPD)+ volunteers. Proliferation was stimulated with mycobacterial antigen (PPD) and evaluated by the incorporation of tritiated thymidine in PBMC cultured with or without 50 μm-vitamin E for 6 d. Cytokine production (IL-2 and interferon (IFN)-γ) was determined by intracellular cytokine staining and by ELISA in the supernatant of PBMC stimulated for 24 h with phytohaemagglutinin or PPD. Our results show that culture with vitamin E increased (P ≤ 0.05) the antigen-induced proliferation of PBMC in TB patients but not in healthy PPD+ volunteers. No significant changes in the number of cytokine-producing cells or in the production of IFN-γ were observed with vitamin E treatment. These results indicate that vitamin E may enhance the antigen-specific in vitro response of PBMC from TB patients.
AB - Micronutrient deficiencies are frequently associated with tuberculosis (TB) worldwide. We tested the effect of exogenous vitamin E on proliferation and cytokine production of peripheral blood mononuclear cells (PBMC) from TB patients and healthy purified protein derivative (PPD)+ volunteers. Proliferation was stimulated with mycobacterial antigen (PPD) and evaluated by the incorporation of tritiated thymidine in PBMC cultured with or without 50 μm-vitamin E for 6 d. Cytokine production (IL-2 and interferon (IFN)-γ) was determined by intracellular cytokine staining and by ELISA in the supernatant of PBMC stimulated for 24 h with phytohaemagglutinin or PPD. Our results show that culture with vitamin E increased (P ≤ 0.05) the antigen-induced proliferation of PBMC in TB patients but not in healthy PPD+ volunteers. No significant changes in the number of cytokine-producing cells or in the production of IFN-γ were observed with vitamin E treatment. These results indicate that vitamin E may enhance the antigen-specific in vitro response of PBMC from TB patients.
KW - Purified protein derivative
KW - Tuberculosis
KW - Vitamin E
UR - http://www.scopus.com/inward/record.url?scp=38049165186&partnerID=8YFLogxK
U2 - 10.1017/S0007114507795302
DO - 10.1017/S0007114507795302
M3 - Artículo
SN - 0007-1145
VL - 99
SP - 224
EP - 229
JO - British Journal of Nutrition
JF - British Journal of Nutrition
IS - 2
ER -