Effect of gamma irradiation doses in the structural and functional properties of mice splenic cells

Yanik Deana, Alexel J. Burgara-Estrella, Maricela Montalvo-Corral, Aracely Angulo-Molina, Mónica A. Acosta-Elías, Erika Silva-Campa, Jose A. Sarabia-Sainz, Iracema C. Rodríguez-Hernández, Martín R. Pedroza-Montero

Research output: Contribution to journalArticlepeer-review


Purpose: Ionizing radiation is nowadays effectively used in cancer treatments. However, the effect of irradiation in immune-system cells is poorly understood and remains controversial. The aim of this work was to determine the effect of γ-irradiation in the structural and functional properties of mice splenic cells. Materials and methods: Structural traits of irradiated splenic cells were evaluated by Atomic Force Microscopy and Raman spectroscopy. Functional properties were measured by gene and protein expression by RT-qPCR and ELISA, respectively. The induced cytotoxic effect was evaluated by MTT assay and the phagocytic capability by flow cytometry. Results: Membrane roughness and molecular composition of splenic adherent cells are not changed by irradiation doses exposure. An increase in transcription of pro-inflammatory cytokines was observed. While protein expression decreased in IL-2 dose-dependent, relevant differences were identified in the anti-inflammatory marker IL-10 at 27 Gy. An increase of cytotoxicity in irradiated cells at 7 Gy and 27 Gy doses was observed, while phagocytosis was slight increased at 7 Gy dose but not statistically significant. Conclusions: We have demonstrated that γ-irradiation affects the splenic cells and changes the cytokines profile toward a pro-inflammatory phenotype and a tendency to increase the cytotoxicity was found, which implies a stimulation of immune response induced by γ-irradiation.

Original languageEnglish
Pages (from-to)286-297
Number of pages12
JournalInternational Journal of Radiation Biology
Issue number3
StatePublished - 6 Mar 2019

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© 2019, © 2019 Taylor & Francis Group LLC.


  • immune response induced
  • splenic cells
  • γ-irradiation


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