TY - JOUR
T1 - Effect of gamma irradiation doses in the structural and functional properties of mice splenic cells
AU - Deana, Yanik
AU - Burgara-Estrella, Alexel J.
AU - Montalvo-Corral, Maricela
AU - Angulo-Molina, Aracely
AU - Acosta-Elías, Mónica A.
AU - Silva-Campa, Erika
AU - Sarabia-Sainz, Jose A.
AU - Rodríguez-Hernández, Iracema C.
AU - Pedroza-Montero, Martín R.
N1 - Publisher Copyright:
© 2019, © 2019 Taylor & Francis Group LLC.
PY - 2019/3/6
Y1 - 2019/3/6
N2 - Purpose: Ionizing radiation is nowadays effectively used in cancer treatments. However, the effect of irradiation in immune-system cells is poorly understood and remains controversial. The aim of this work was to determine the effect of γ-irradiation in the structural and functional properties of mice splenic cells. Materials and methods: Structural traits of irradiated splenic cells were evaluated by Atomic Force Microscopy and Raman spectroscopy. Functional properties were measured by gene and protein expression by RT-qPCR and ELISA, respectively. The induced cytotoxic effect was evaluated by MTT assay and the phagocytic capability by flow cytometry. Results: Membrane roughness and molecular composition of splenic adherent cells are not changed by irradiation doses exposure. An increase in transcription of pro-inflammatory cytokines was observed. While protein expression decreased in IL-2 dose-dependent, relevant differences were identified in the anti-inflammatory marker IL-10 at 27 Gy. An increase of cytotoxicity in irradiated cells at 7 Gy and 27 Gy doses was observed, while phagocytosis was slight increased at 7 Gy dose but not statistically significant. Conclusions: We have demonstrated that γ-irradiation affects the splenic cells and changes the cytokines profile toward a pro-inflammatory phenotype and a tendency to increase the cytotoxicity was found, which implies a stimulation of immune response induced by γ-irradiation.
AB - Purpose: Ionizing radiation is nowadays effectively used in cancer treatments. However, the effect of irradiation in immune-system cells is poorly understood and remains controversial. The aim of this work was to determine the effect of γ-irradiation in the structural and functional properties of mice splenic cells. Materials and methods: Structural traits of irradiated splenic cells were evaluated by Atomic Force Microscopy and Raman spectroscopy. Functional properties were measured by gene and protein expression by RT-qPCR and ELISA, respectively. The induced cytotoxic effect was evaluated by MTT assay and the phagocytic capability by flow cytometry. Results: Membrane roughness and molecular composition of splenic adherent cells are not changed by irradiation doses exposure. An increase in transcription of pro-inflammatory cytokines was observed. While protein expression decreased in IL-2 dose-dependent, relevant differences were identified in the anti-inflammatory marker IL-10 at 27 Gy. An increase of cytotoxicity in irradiated cells at 7 Gy and 27 Gy doses was observed, while phagocytosis was slight increased at 7 Gy dose but not statistically significant. Conclusions: We have demonstrated that γ-irradiation affects the splenic cells and changes the cytokines profile toward a pro-inflammatory phenotype and a tendency to increase the cytotoxicity was found, which implies a stimulation of immune response induced by γ-irradiation.
KW - immune response induced
KW - splenic cells
KW - γ-irradiation
UR - https://doi.org/10.1080/09553002.2019.1547435
U2 - 10.1080/09553002.2019.1547435
DO - 10.1080/09553002.2019.1547435
M3 - Artículo
C2 - 30496016
SN - 0955-3002
VL - 95
SP - 286
EP - 297
JO - International Journal of Radiation Biology
JF - International Journal of Radiation Biology
IS - 3
ER -