TY - JOUR
T1 - Effect of pulsed ultrasound on the physicochemical characteristics and emulsifying properties of squid (Dosidicus gigas) mantle proteins
AU - Higuera-Barraza, O. A.
AU - Torres-Arreola, W.
AU - Ezquerra-Brauer, J. M.
AU - Cinco-Moroyoqui, F. J.
AU - Rodríguez Figueroa, J. C.
AU - Marquez-Ríos, E.
N1 - Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2017/9
Y1 - 2017/9
N2 - Food technologists are always looking to improve the functional properties of proteins. In this sense, in last years ultrasound has been used to improve some functional properties. For this reason, and considering that jumbo squid is an important fishery in northwest Mexico, the purpose of this research was to determine the effect of pulsed ultrasound on the physicochemical characteristics and emulsifying properties of squid (Dosidicus gigas) mantle proteins. Pulsed ultrasound (20 kHz, 20, and 40% amplitude) was applied for 30, 60, and 90 s to a protein extract prepared from giant squid mantle causing an increase (p < 0.05) in surface hydrophobicity (So) from 108.4 ± 1.4 to 239.1 ± 2.4 after application of pulsed ultrasound at 40% of amplitude for 90 s. The electrophoretic profile and the total and reactive sulfhydryl contents were not affected (p ⩾ 0.05) by the ultrasound treatment. The emulsifying ability of the protein solution was improved (p < 0.05), whereas the Emulsifier Activity Index (EAI) varied from123.67 ± 5.52 m2/g for the control and increased up to 217.7 ± 3.8 m2/g after application of the ultrasound. The Stability Emulsifier Index (EEI) was improved at 40% of amplitude by 60 and 90 s. The results suggested that pulsed ultrasound used as pretreatment induced conformational changes in giant squid proteins, which improved the interfacial association between protein-oil phases, thus contributing to the improvement of their emulsifient properties.
AB - Food technologists are always looking to improve the functional properties of proteins. In this sense, in last years ultrasound has been used to improve some functional properties. For this reason, and considering that jumbo squid is an important fishery in northwest Mexico, the purpose of this research was to determine the effect of pulsed ultrasound on the physicochemical characteristics and emulsifying properties of squid (Dosidicus gigas) mantle proteins. Pulsed ultrasound (20 kHz, 20, and 40% amplitude) was applied for 30, 60, and 90 s to a protein extract prepared from giant squid mantle causing an increase (p < 0.05) in surface hydrophobicity (So) from 108.4 ± 1.4 to 239.1 ± 2.4 after application of pulsed ultrasound at 40% of amplitude for 90 s. The electrophoretic profile and the total and reactive sulfhydryl contents were not affected (p ⩾ 0.05) by the ultrasound treatment. The emulsifying ability of the protein solution was improved (p < 0.05), whereas the Emulsifier Activity Index (EAI) varied from123.67 ± 5.52 m2/g for the control and increased up to 217.7 ± 3.8 m2/g after application of the ultrasound. The Stability Emulsifier Index (EEI) was improved at 40% of amplitude by 60 and 90 s. The results suggested that pulsed ultrasound used as pretreatment induced conformational changes in giant squid proteins, which improved the interfacial association between protein-oil phases, thus contributing to the improvement of their emulsifient properties.
KW - Emulsifying ability
KW - Functional properties
KW - Proteins
KW - Pulsed ultrasound
UR - http://www.scopus.com/inward/record.url?scp=85009786889&partnerID=8YFLogxK
U2 - 10.1016/j.ultsonch.2017.01.008
DO - 10.1016/j.ultsonch.2017.01.008
M3 - Artículo
SN - 1350-4177
VL - 38
SP - 829
EP - 834
JO - Ultrasonics Sonochemistry
JF - Ultrasonics Sonochemistry
ER -