Effect of the deficiency of nitrate and silicate on the growth and composition of the benthic diatom naviculaincerta

José Jesús Encinas-Arzate, Enrique Marquez-Ríos, José Antonio López-Elías*, Wilfrido Torres-Areola, José Ángel Huerta-Ocampo, Juan Carlos Ramírez-Suárez

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Naviculaincerta is a benthic diatom with potential use in nutrition and health for humans and aquaculture. Therefore, it is important to know its optimal growth conditions and biochemical composition. In this study, the effects of nitrate and silicate concentration on the growth kinetics and biochemical composition of N. incerta under laboratory conditions were measured. F/2 media was used as the control, and different concentrations of nitrogen (N/4, N/8 and N/16) and silicates (Si/4, Si/8 and Si/16) were evaluated. We measured cell concentration, chlorophyll-a and conducted proximal chemical analyses. It was found that different concentrations of nutrients affected the diatom’s growth kinetics and affected the concentrations of ash, chlorophyll, protein, lipids and carbohydrates. The highest concentration of lipids was obtained in the limiting treatment of nitrogen N/8 (27.09%), while the lowest value was found with silicate Si/8 media (16.97%). Carbohydrates increased compared to the control, with the N/16 treatment presenting the maximal concentration (23.31%). Treatments with reduced nitrate (N/8 and N/16) demonstrated the lowest concentrations of protein (18.75 and 12.44%, respectively), while in reduced silicate treatments, no statistical differences (P ≥ 0.05) were observed. Therefore, media limited nitrogen and silicates affected the growth kinetics and proximal chemical composition of N. incerta. The growth of this species using the N/8 medium is a suitable method for increasing lipid concentration in N. incerta.

Original languageEnglish
Pages (from-to)280-286
Number of pages7
JournalLatin American Journal of Aquatic Research
Issue number2
StatePublished - 2020

Bibliographical note

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© 2020, Escuela de Ciencias del Mar. All rights reserved.


  • Chlorophyll
  • Growth
  • Lipid production
  • Naviculaincerta
  • Nutrient limiting
  • Proximal composition
  • Stress response


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