TY - JOUR
T1 - Effect of ultrafiltration of Pitaya extract (Stenocereus thurberi) on Its phytochemical content, antioxidant capacity, and UPLC-DAD-MS profile
AU - Castro-Enríquez, Daniela D.
AU - Montaño-Leyva, Beatriz
AU - Del Toro-Sánchez, Carmen L.
AU - Juárez-Onofre, Josué E.
AU - Carvajal-Millán, Elizabeth
AU - López-Ahumada, Guadalupe A.
AU - Barreras-Urbina, Carlos G.
AU - Tapia-Hernández, José A.
AU - Rodríguez-Félix, Francisco
N1 - Publisher Copyright:
© 2020 by the authors.
PY - 2020/1/1
Y1 - 2020/1/1
N2 - Stenocereus thurberi is an endemic species in northwestern Mexico. It produces colorful fruits called pitayas that have an edible pulp. They have phytochemical compounds associated with biological activities. Ultrafiltration is a widely used method for the clarification of fruit juices and the recovery of phytochemicals. However, its effect has not been extensively studied in extracts. Therefore, the objective of this work is to study the effect of the ultrafiltration of pitaya extract (Stenocereus thurberi) on its phytochemical content, antioxidant capacity, and identification of phenolic compounds by UPLC-DAD-MS, providing greater knowledge about the pitaya. In this study, two extracts were analyzed, the unclarified extract (UE) and the clarified extract (CE). The antioxidant capacity was higher in the CE with 15.93 ± 0.42 mM TE/g, DPPH and 18.37 ± 0.016 mM TE/g, ABTS. The UPLC-MS analysis indicated the decrease in phenolic compounds in the CE and the presence of gallic acid and resorcinol, compounds that had not been identified in other species of Stenocereus spp. The correlation analysis indicated that all the phytochemicals present in the pitaya contribute significantly to the antioxidant capacity. The ultrafiltration process could be a viable option to improve the biological activity of the natural extracts.
AB - Stenocereus thurberi is an endemic species in northwestern Mexico. It produces colorful fruits called pitayas that have an edible pulp. They have phytochemical compounds associated with biological activities. Ultrafiltration is a widely used method for the clarification of fruit juices and the recovery of phytochemicals. However, its effect has not been extensively studied in extracts. Therefore, the objective of this work is to study the effect of the ultrafiltration of pitaya extract (Stenocereus thurberi) on its phytochemical content, antioxidant capacity, and identification of phenolic compounds by UPLC-DAD-MS, providing greater knowledge about the pitaya. In this study, two extracts were analyzed, the unclarified extract (UE) and the clarified extract (CE). The antioxidant capacity was higher in the CE with 15.93 ± 0.42 mM TE/g, DPPH and 18.37 ± 0.016 mM TE/g, ABTS. The UPLC-MS analysis indicated the decrease in phenolic compounds in the CE and the presence of gallic acid and resorcinol, compounds that had not been identified in other species of Stenocereus spp. The correlation analysis indicated that all the phytochemicals present in the pitaya contribute significantly to the antioxidant capacity. The ultrafiltration process could be a viable option to improve the biological activity of the natural extracts.
KW - Antioxidant capacity
KW - Phytochemical
KW - Pitaya
KW - Stenocereus thurberi
KW - Ultrafiltration
KW - UPLC-DAD-MS
UR - http://www.scopus.com/inward/record.url?scp=85077872290&partnerID=8YFLogxK
U2 - 10.3390/molecules25020281
DO - 10.3390/molecules25020281
M3 - Artículo
C2 - 31936683
AN - SCOPUS:85077872290
SN - 1420-3049
VL - 25
JO - Molecules
JF - Molecules
IS - 2
M1 - 281
ER -