Effect on Human Vascular Endothelial Cells of Au Nanoparticles Synthesized fromVitex mollis

Abraham Arizmendi-Grijalva; Aarón Alberto Martínez-Higuera; Jesús Adriana Soto-Guzmán; Juan Manuel Martínez-Soto; Ericka Rodríguez-León; César Rodríguez-Beas; Luis Fernando López-Soto; Francisco Javier Alvarez-Cirerol; Nadia Garcia-Flores; Pedro Cortés-Reynosa; Eduardo Pérez-Salazar; Ramón Iñiguez-Palomares

doi:10.1021/acsomega.1c01506

Effect on Human Vascular Endothelial Cells of Au Nanoparticles Synthesized fromVitex mollis

Abraham Arizmendi-Grijalva, Aarón Alberto Martínez-Higuera, Jesús Adriana Soto-Guzmán, Juan Manuel Martínez-Soto, Ericka Rodríguez-León, César Rodríguez-Beas, Luis Fernando López-Soto, Francisco Javier Alvarez-Cirerol, Nadia Garcia-Flores, Pedro Cortés-Reynosa, Eduardo Pérez-Salazar, Ramón Iñiguez-Palomares^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

A green method for synthesizing gold nanoparticles is proposed using hydroethanolic extract ofVitex mollisfruit (Vm extract) as a reducer and stabilizer. The formation of gold nanoparticles synthesized with Vm extract (AuVmNPs) was monitored by measuring the ultraviolet-visible spectra. The morphology and crystalline phase were determined using scanning electron microscopy, X-ray diffraction, and high-resolution transmission electron microscopy. Synthesized nanoparticles were generally spherical, and the size distribution obtained by transmission electron microscopy shows two populations with mean sizes of 12.5 and 22.5 nm. Cell viability assay using MTT and cellular apoptosis studies using annexin V on human umbilical vein endothelial cells (HUVECs) and the human mammary epithelial cell line (MCF10A) indicate that AuVmNPs have low toxicity. Cell migration tests indicate that AuVmNPs significantly inhibit HUVEC cell migration in a dose-dependent manner. The evaluation of the localization of AuVmNPs in HUVECs using confocal laser scanning microscopy indicates that nanoparticles penetrate cells and are found in the cytosol without preferential distribution and without entering the nucleus. The inhibitory effect on cellular migration and low toxicity suggest AuVmNPs as appropriate candidates in future studies of antiangiogenic activity.

Original language	English
Pages (from-to)	24338-24350
Number of pages	13
Journal	ACS Omega
Volume	6
Issue number	38
DOIs	https://doi.org/10.1021/acsomega.1c01506
State	Published - 28 Sep 2021

Bibliographical note

Funding Information:
TEM analysis was supported by the Scanning Electron Microscopy Lab of the Physics Department. All authors want to acknowledge the Biomaterials Laboratory for the use of a confocal microscope of the Physics Department, University of Sonora. A.A.-G. would like to thank Conacyt for Doctoral Fellowship number 748633, and A.A.M.-H. would like to thank Conacyt for Post-Doctoral Fellowship (2019-000019-01NACV-00449), support number 740180.

Publisher Copyright:
© 2021 The Authors. Published by American Chemical Society

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Arizmendi-Grijalva, A., Martínez-Higuera, A. A., Soto-Guzmán, J. A., Martínez-Soto, J. M., Rodríguez-León, E., Rodríguez-Beas, C., López-Soto, L. F., Alvarez-Cirerol, F. J., Garcia-Flores, N., Cortés-Reynosa, P., Pérez-Salazar, E., & Iñiguez-Palomares, R. (2021). Effect on Human Vascular Endothelial Cells of Au Nanoparticles Synthesized fromVitex mollis. ACS Omega, 6(38), 24338-24350. https://doi.org/10.1021/acsomega.1c01506

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title = "Effect on Human Vascular Endothelial Cells of Au Nanoparticles Synthesized fromVitex mollis",

abstract = "A green method for synthesizing gold nanoparticles is proposed using hydroethanolic extract ofVitex mollisfruit (Vm extract) as a reducer and stabilizer. The formation of gold nanoparticles synthesized with Vm extract (AuVmNPs) was monitored by measuring the ultraviolet-visible spectra. The morphology and crystalline phase were determined using scanning electron microscopy, X-ray diffraction, and high-resolution transmission electron microscopy. Synthesized nanoparticles were generally spherical, and the size distribution obtained by transmission electron microscopy shows two populations with mean sizes of 12.5 and 22.5 nm. Cell viability assay using MTT and cellular apoptosis studies using annexin V on human umbilical vein endothelial cells (HUVECs) and the human mammary epithelial cell line (MCF10A) indicate that AuVmNPs have low toxicity. Cell migration tests indicate that AuVmNPs significantly inhibit HUVEC cell migration in a dose-dependent manner. The evaluation of the localization of AuVmNPs in HUVECs using confocal laser scanning microscopy indicates that nanoparticles penetrate cells and are found in the cytosol without preferential distribution and without entering the nucleus. The inhibitory effect on cellular migration and low toxicity suggest AuVmNPs as appropriate candidates in future studies of antiangiogenic activity.",

author = "Abraham Arizmendi-Grijalva and Mart{\'i}nez-Higuera, {Aar{\'o}n Alberto} and Soto-Guzm{\'a}n, {Jes{\'u}s Adriana} and Mart{\'i}nez-Soto, {Juan Manuel} and Ericka Rodr{\'i}guez-Le{\'o}n and C{\'e}sar Rodr{\'i}guez-Beas and L{\'o}pez-Soto, {Luis Fernando} and Alvarez-Cirerol, {Francisco Javier} and Nadia Garcia-Flores and Pedro Cort{\'e}s-Reynosa and Eduardo P{\'e}rez-Salazar and Ram{\'o}n I{\~n}iguez-Palomares",

note = "Publisher Copyright: {\textcopyright} 2021 The Authors. Published by American Chemical Society",

year = "2021",

month = sep,

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Arizmendi-Grijalva, A, Martínez-Higuera, AA, Soto-Guzmán, JA, Martínez-Soto, JM, Rodríguez-León, E, Rodríguez-Beas, C, López-Soto, LF, Alvarez-Cirerol, FJ, Garcia-Flores, N, Cortés-Reynosa, P, Pérez-Salazar, E & Iñiguez-Palomares, R 2021, 'Effect on Human Vascular Endothelial Cells of Au Nanoparticles Synthesized fromVitex mollis', ACS Omega, vol. 6, no. 38, pp. 24338-24350. https://doi.org/10.1021/acsomega.1c01506

TY - JOUR

T1 - Effect on Human Vascular Endothelial Cells of Au Nanoparticles Synthesized fromVitex mollis

AU - Arizmendi-Grijalva, Abraham

AU - Martínez-Higuera, Aarón Alberto

AU - Soto-Guzmán, Jesús Adriana

AU - Martínez-Soto, Juan Manuel

AU - Rodríguez-León, Ericka

AU - Rodríguez-Beas, César

AU - López-Soto, Luis Fernando

AU - Alvarez-Cirerol, Francisco Javier

AU - Garcia-Flores, Nadia

AU - Cortés-Reynosa, Pedro

AU - Pérez-Salazar, Eduardo

AU - Iñiguez-Palomares, Ramón

PY - 2021/9/28

Y1 - 2021/9/28

N2 - A green method for synthesizing gold nanoparticles is proposed using hydroethanolic extract ofVitex mollisfruit (Vm extract) as a reducer and stabilizer. The formation of gold nanoparticles synthesized with Vm extract (AuVmNPs) was monitored by measuring the ultraviolet-visible spectra. The morphology and crystalline phase were determined using scanning electron microscopy, X-ray diffraction, and high-resolution transmission electron microscopy. Synthesized nanoparticles were generally spherical, and the size distribution obtained by transmission electron microscopy shows two populations with mean sizes of 12.5 and 22.5 nm. Cell viability assay using MTT and cellular apoptosis studies using annexin V on human umbilical vein endothelial cells (HUVECs) and the human mammary epithelial cell line (MCF10A) indicate that AuVmNPs have low toxicity. Cell migration tests indicate that AuVmNPs significantly inhibit HUVEC cell migration in a dose-dependent manner. The evaluation of the localization of AuVmNPs in HUVECs using confocal laser scanning microscopy indicates that nanoparticles penetrate cells and are found in the cytosol without preferential distribution and without entering the nucleus. The inhibitory effect on cellular migration and low toxicity suggest AuVmNPs as appropriate candidates in future studies of antiangiogenic activity.

AB - A green method for synthesizing gold nanoparticles is proposed using hydroethanolic extract ofVitex mollisfruit (Vm extract) as a reducer and stabilizer. The formation of gold nanoparticles synthesized with Vm extract (AuVmNPs) was monitored by measuring the ultraviolet-visible spectra. The morphology and crystalline phase were determined using scanning electron microscopy, X-ray diffraction, and high-resolution transmission electron microscopy. Synthesized nanoparticles were generally spherical, and the size distribution obtained by transmission electron microscopy shows two populations with mean sizes of 12.5 and 22.5 nm. Cell viability assay using MTT and cellular apoptosis studies using annexin V on human umbilical vein endothelial cells (HUVECs) and the human mammary epithelial cell line (MCF10A) indicate that AuVmNPs have low toxicity. Cell migration tests indicate that AuVmNPs significantly inhibit HUVEC cell migration in a dose-dependent manner. The evaluation of the localization of AuVmNPs in HUVECs using confocal laser scanning microscopy indicates that nanoparticles penetrate cells and are found in the cytosol without preferential distribution and without entering the nucleus. The inhibitory effect on cellular migration and low toxicity suggest AuVmNPs as appropriate candidates in future studies of antiangiogenic activity.

UR - http://www.scopus.com/inward/record.url?scp=85115992028&partnerID=8YFLogxK

U2 - 10.1021/acsomega.1c01506

DO - 10.1021/acsomega.1c01506

M3 - Artículo

C2 - 34604617

AN - SCOPUS:85115992028

SN - 2470-1343

VL - 6

SP - 24338

EP - 24350

JO - ACS Omega

JF - ACS Omega

IS - 38

ER -

Effect on Human Vascular Endothelial Cells of Au Nanoparticles Synthesized fromVitex mollis

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