Effect on Human Vascular Endothelial Cells of Au Nanoparticles Synthesized fromVitex mollis

Abraham Arizmendi-Grijalva, Aarón Alberto Martínez-Higuera, Jesús Adriana Soto-Guzmán, Juan Manuel Martínez-Soto, Ericka Rodríguez-León, César Rodríguez-Beas, Luis Fernando López-Soto, Francisco Javier Alvarez-Cirerol, Nadia Garcia-Flores, Pedro Cortés-Reynosa, Eduardo Pérez-Salazar, Ramón Iñiguez-Palomares*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


A green method for synthesizing gold nanoparticles is proposed using hydroethanolic extract ofVitex mollisfruit (Vm extract) as a reducer and stabilizer. The formation of gold nanoparticles synthesized with Vm extract (AuVmNPs) was monitored by measuring the ultraviolet-visible spectra. The morphology and crystalline phase were determined using scanning electron microscopy, X-ray diffraction, and high-resolution transmission electron microscopy. Synthesized nanoparticles were generally spherical, and the size distribution obtained by transmission electron microscopy shows two populations with mean sizes of 12.5 and 22.5 nm. Cell viability assay using MTT and cellular apoptosis studies using annexin V on human umbilical vein endothelial cells (HUVECs) and the human mammary epithelial cell line (MCF10A) indicate that AuVmNPs have low toxicity. Cell migration tests indicate that AuVmNPs significantly inhibit HUVEC cell migration in a dose-dependent manner. The evaluation of the localization of AuVmNPs in HUVECs using confocal laser scanning microscopy indicates that nanoparticles penetrate cells and are found in the cytosol without preferential distribution and without entering the nucleus. The inhibitory effect on cellular migration and low toxicity suggest AuVmNPs as appropriate candidates in future studies of antiangiogenic activity.

Original languageEnglish
Pages (from-to)24338-24350
Number of pages13
JournalACS Omega
Issue number38
StatePublished - 28 Sep 2021

Bibliographical note

Funding Information:
TEM analysis was supported by the Scanning Electron Microscopy Lab of the Physics Department. All authors want to acknowledge the Biomaterials Laboratory for the use of a confocal microscope of the Physics Department, University of Sonora. A.A.-G. would like to thank Conacyt for Doctoral Fellowship number 748633, and A.A.M.-H. would like to thank Conacyt for Post-Doctoral Fellowship (2019-000019-01NACV-00449), support number 740180.

Publisher Copyright:
© 2021 The Authors. Published by American Chemical Society


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